Anti-acetyl-Histone H3 (Ac-Lys9) antibody, Mouse monoclonal
SIGMA/H0913 - clone AH3-120, purified from hybridoma cell culture
Synonym: Anti-H3K9ac
MDL Number: MFCD02685921
Product Type: Chemical
Immunoblotting Histone fraction isolated from HeLa cells was separated on SDS-PAGE and probed with 1 μg/mL Monoclonal Anti-acetyl-Histone H3 (Ac-Lys9) Clone: AH3-120 (Cat. No. H0913). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | AH3-120, monoclonal |
| concentration | ~2 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen ~17 kDa |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, bovine, Caenorhabditis elegans, frog, Drosophila, chicken, rat, mouse |
| storage temp. | −20°C |
| target post-translational modification | acetylation (Lys9) |
| technique(s) | immunocytochemistry: suitable |
| indirect ELISA: suitable | |
| microarray: suitable | |
| western blot: 1-2 μg/mL using whole cell extract of mouse fibroblasts 3T3 cell line treated with sodium butyrate | |
| UniProt accession no. | P68431  |
| Application: | Monoclonal Anti-acetyl-Histone H3 (Ac-Lys9) antibody may be used in various applications including ELISA, immunoblotting (approx. 17 kDa), and immunocytochemistry. |
| Biochem/physiol Actions: | Acetyl-Histone H3 hav Acetylation of lysine residues within these N-terminal domains of histones by histone acetyl-transferase (HATs), including Gcn5p, PCAF, p300/CBP and TAFII250 is associated with transcriptional activation. This modification results in remodeling of the nucleosome structure into an open conformation more accessible to transcription complexes. Conversely, histone deacetylation by histone deacetylase (HDACs) is associated with transcription repression reversing the chromatin remodeling process. In most species, histone H3 is primarily acetylated at lysine 9, 14, 18, and 23. Acetylation at lysine 9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms. Acetylation of specific lysines in histone H3 is also associated with processes apart from transcription. During DNA replication, new histones are rapidly synthesized and assembled into replicated DNA. Histones H3 and H4 are brought to replicating chromatin in a pre-acetylated state that turns into a de-acetylated state after replication is completed and the newly assembled chromatin matures. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Acetyl-Histone H3 (Ac-Lys9) recognizes human histone H3 when acetylated on Lys9. Staining of the histone H3-Ac-Lys9 band in immunoblotting is specifically inhibited with the acetylated histone H3 immunizing peptide but not with the nonacetylated one. |
| Immunogen: | synthetic, acetylated histone H3 peptide (amino acids 7-20, Ac-Lys9) corresponding to the N-terminus of human histone H3. The sequence is identical in many species including mouse, rat, bovine, chicken, frog, Drosophila, and C. elegans, and is highly conserved (single amino acid substitution) in Tetrahymena histone H3. |
| Packaging: | 200 μL in glass bottle |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |