Hanks’ Balanced Salts
SIGMA/H1387 - Modified, without phenol red and sodium bicarbonate, powder, suitable for cell culture
Synonym: HBSS
MDL Number: MFCD00217418
Product Type: Chemical
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| form | powder |
| Quality Level | 500  |
| storage temp. | 2-8°C |
| technique(s) | cell culture | mammalian: suitable |
| Application: | Hanks′ Balanced Salts has been used: • for diluting digested tissues during isolation of pancreatic blood vessels • for washing mouse intestinal mucosal cells before lysis and sonication • as an immersion solution to test the in vitro bioactivity of poly(ε-caprolactone)-coat |
| General description: | Balanced salt solution (BSS) serves as an irrigating, transporting, and diluting fluid while maintaining intra- and extracellular osmotic balance. It also provides cells with water and certain bulk inorganic ions essential for normal cell metabolism. BSS when combined with a carbohydrate, such as glucose, provides the principal energy source for cell metabolism. In addition, it also provides a buffering system to maintain the medium within the physiological pH range (7.2-7.6). |
| Other Notes: | The pH is adjusted after addition of sodium bicarbonate. Investigators who work with stem cells or cell clones frequently prefer media without phenol red. |
| Preparation Note: | Formulated to contain 9.7 grams of powder per liter of medium. |
| Preparation Note: | Supplement with 0.35 g/L sodium bicarbonate. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352207 |