3α-Hydroxysteroid Dehydrogenase from Pseudomonas testosteroni
SIGMA/H1506 - ≥15 units/mg protein
Synonym: 3α-Hydroxysteroid:NAD(P)+ oxidoreductase
CAS Number: 9028-56-2
EC Number: 232-839-5
MDL Number: MFCD00130298
Product Type: Chemical
Unit Definition One unit will oxidize 1.0 μmole of androsterone per min at pH 8.9 at 25 °C in the presence of β-NAD+.
| biological source | bacterial (Pseudomonas testosteroni) |
| composition | Protein, ≥25% |
| foreign activity | β-hydroxysteroid dehydrogenase ≤0.5% |
| alcohol dehydrogenase ≤0.05% | |
| form | lyophilized solid |
| purified by | chromatography |
| Quality Level | 200  |
| specific activity | ≥15 units/mg protein |
| storage temp. | −20°C |
| Application: | 3α-Hydroxysteroid Dehydrogenase from Pseudomonas testosterone has been used: • in the preparation of composite electrode platform to for the determination of androsterone • in potassium phosphate buffer for the bioluminescent assay of bile acids in serum • for total bile salts assay using an enzymatic method |
| Biochem/physiol Actions: | 3α-hydroxysteroid dehydrogenase (3α-HSD) may help to modulate the occupancy of the androgen receptor (AR) in androgen target tissues. It may also act in hindering androgen action. |
| Other Notes: | One unit will oxidize 1.0 μmole of androsterone per min at pH 8.9 at 25 °C in the presence of β-NAD+. |
| Packaging: | 50 units in glass bottle |
| Physical form: | Lyophilized powder containing potassium phosphate buffer salt, EDTA, and DL-dithiothreitol |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥15 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 1.1.1.50 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |