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Anti-Hamster IgG (whole molecule) antibody produced in rabbit

SIGMA/H1643 - affinity isolated antibody, buffered aqueous solution

MDL Number: MFCD00162371
Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-H1643-1ML 1 mL
$236.00
1/EA
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ELISA Anti-Hamster IgG (whole molecule) antibody produced in Rabbit (Cat. No. H1643), followed by 1:5,000 Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat (Cat. No. A0545), was serially diluted to detect Hamster IgG by indirect ELISA.

 

antibody form affinity isolated antibody
antibody product type secondary antibodies
biological source rabbit
clone polyclonal
conjugate unconjugated
form buffered aqueous solution
Quality Level 200 
shipped in dry ice
storage temp. −20°C
target post-translational modification unmodified
technique(s) indirect ELISA: 1:100,000
Application: Anti-Hamster IgG (whole molecule) antibody produced in rabbit is suitable for use in Immunoelectron Microscopy and FACS.
Biochem/physiol Actions: IgG antibody subtype is the most abundant serum immunoglobulin of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections.
Biochem/physiol Actions: IgG, a monoclonal antibody can be cleaved at the hinge region by nonspecific proteases like papain and pepsin. This can result in univalent Fab (Fragment antigen binding) fragments or bivalent F(ab′)2 fragments. These two enzymes have a broad substrate specificity resulting in heterogenous fragments.
Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description: IgG is present in large quantities in the human serum. IgG is composed of glycoproteins, out of which it is 82-96% proteins and 4-18% carbohydrates. It consists of four sub-classes i.e IgG1, IgG2, IgG3, and IgG4. IgG is composed of four polypeptide chains-two heavy chains (γ chains) and two light chains (κ or λ chains) which are linked by inter-chain disulfide bonds. The heavy chains consist of a N-terminal variable domain (VH) and three constant domains (CH1, CH2, CH3). A hinge region exists between the CH1 and CH2 region. The light chains have one N-terminal variable domain (VL) and one constant domain (CL). The heavy and the light chains are linked at VH and CH1 domain to form the Fab arm (Fragment antigen binding). The antigen binds to the V regions of the antibody.
Physical form: Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as preservative
Hazard Codes Xn
Risk Statements 21/22
Safety Statements 36/37-60
RIDADR NONH for all modes of transport
WGK Germany nwg
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Storage Temp. −20°C
UNSPSC 12352203

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