Monoclonal Anti-HLA Class I Antigen antibody produced in mouse
SIGMA/H1650 - clone W6/32, purified immunoglobulin, buffered aqueous solution
Synonym: Monoclonal Anti-HLA Class I Antigen
Product Type: Chemical
Immunohistochemistry Formalin-fixed, paraffin-embedded heat retrieval tissue section of human tonsil stained with 1:10 Monoclonal Monoclonal Anti-HLA Class I Antigen antibody produced in Mouse clone W6/32 (Cat. No. H1650) followed by Anti-Mouse IgG (Fab specific)-FITC antibody produced in Goat (Cat. No. F5262).
Flow Cytometry 1×106 Human lymphocytes were labeled with 5 μg/test Anti-HLA Class I Antigen antibody Mouse monoclonal, Clone: W6/32 (Cat. No. H1650) followed by Rabbit Anti-Mouse IgG, FITC conjugate. Yellow histogram: negative control without first antibody; Orange histogram: antibody specific staining.
Flow Cytometry 1×106 Human monocytes were labeled with 5 μg/test Anti-HLA Class I Antigen antibody Mouse monoclonal, Clone: W6/32 (Cat. No. H1650) followed by Rabbit Anti-Mouse IgG, FITC conjugate. Yellow histogram: negative control without first antibody; Orange histogram: antibody specific staining.
Flow Cytometry 1×106 Human granulocytes were labeled with 5 μg/test Anti-HLA Class I Antigen antibody, Mouse monoclonal, Clone: W6/32 (Cat. No. H1650) followed by Rabbit Anti-Mouse IgG, FITC conjugate. Yellow histogram: negative control without first antibody; Orange histogram: antibody specific staining.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | W6/32, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2a |
| Quality Level | 200  |
| shipped in | wet ice |
| species reactivity | monkey, human |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | flow cytometry: 5 μL using 1 × 106 cells |
| immunoprecipitation (IP): suitable | |
| indirect immunofluorescence: 1:10 using acetone-fixed human tonsil frozen sections. | |
| UniProt accession no. | P04439  |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Immunofluorescence (1 paper) |
| Biochem/physiol Actions: | HLA class I molecules like HLA-A, -B and −C help in the presentation of endogenous antigen to CD8+ (cluster of differentiation 8) T cells. |
| Biochem/physiol Actions: | Recognizes the human HLA Class I antigen expressed on most human nucleated cells. The antibody reacts with a monomorphic epitope on the 44-45 kDa α-chain polypeptide of human class I HLA molecules (HLA-A,B,C). The antibody may be used in immunoprecipitation, complement mediated cytotoxicity, flow cytometry and immunohistochemical staining of acetone-fixed, frozen sections and cell smears. It cross-reacts with cells from African and Asian apes and old world monkeys. No reactivity is seen with cells from most new world monkeys or from non-primates. The epitope recognized by this antibody is sensitive to routine formalin fixation and paraffin embedding. 4th Workshop: code no. P1 5th Workshop: code nos. BP166, BP288, BP407, B 005 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Class I proteins are highly polymorphic, in which HLA-B seems to be the most polymorphic gene in the human genome. HLA-A, -B, -C are classical class I proteins and are heterodimers. HLA-A, -B, -C consists of a polymorphic transmembrane α heavy chain and an invariant water-soluble light chain. HLA-B is located on human chromosome 6p21. The previously assigned protein identifier P01891 has been merged into P04439. Full details can be found on the UniProt database. |
| Immunogen: | human tonsil leukocyte membrane preparation. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |