(+)-Biotin N-hydroxysuccinimide ester

SIGMA/H1759 - ≥98% (HPLC), powder

Synonym: (+)-Biotin N-succinimidyl ester; d-Biotin NHS ester; BNHS; Biotinyl-N-hydroxy-succinimide; N-Hydroxysuccinimidobiotin; N-Succinimidyl D-biotinate; NHS-Biotin

CAS Number: 35013-72-0
Empirical Formula (Hill Notation): C₁₄H₁₉N₃O₅S
Molecular Weight: 341.38
MDL Number: MFCD00078531
Linear Formula: C₁₄H₁₉N₃O₅S
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-H1759-5MG	5 mg	$29.50 1/EA	Add To Favorites
45-H1759-25MG	25 mg	$78.90 1/EA	Add To Favorites
45-H1759-100MG	100 mg	$215.00 1/EA	Add To Favorites
45-H1759-250MG	250 mg	$379.00 1/EA	Add To Favorites
45-H1759-1G	1 g	$1110.00 1/EA	Add To Favorites

ELISA assay utilizing a biotinylated antibody and streptavidin-peroxidase detection.

Properties
Descriptions
Safety Info.
Basic Data

assay	≥98% (HPLC)
color	white to off-white
form	powder
InChI	1S/C14H19N3O5S/c18-10-5-6-11(19)17(10)22-12(20)4-2-1-3-9-13-8(7-23-9)15-14(21)16-13/h8-9,13H,1-7H2,(H2,15,16,21)/t8-,9-,13-/m0/s1
InChI key	YMXHPSHLTSZXKH-RVBZMBCESA-N
mp	212 °C
Quality Level	200
reaction suitability	reaction type: Biotinylations
SMILES string	[H][C@]12CS[C@@H](CCCCC(=O)ON3C(=O)CCC3=O)[C@@]1([H])NC(=O)N2
solubility	DMF: ≤50 mg/mL (Stable for at least one month in dry DMF)
	DMSO: soluble
storage temp.	−20°C

Application:	(+) Biotin NHS ester or (+)-Biotin N-hydroxysuccinimide ester has been used: • to biotinylate Hyb 246-4 for surfactant protein D enzyme-linked immunosorbent assay (SP-D ELISA) • to biotinylate proteins to form preformed complexes • to label and modify monoclonal anti-microfibrillar-associated protein 4 (MFAP4) (HG-HYB 7-18) to bind to alphaLISA streptavidin-coated donor beads
Biochem/physiol Actions:	The N-hydroxysuccinimide ester (NHS)-biotin modification helps in the biotinylation of the target lysine residue at its ε-amino group and identifies the surface lysine residues. It is used as a tool to identify the interaction of proteins with several biological molecules, like protein-polysaccharide interactions, protein-protein interactions, protein-nucleic acid interactions, and protein-ligand interactions. NHS-biotin may be used in the biotinylation of proteins and peptides. It is typically coupled to primary amine in the pH range of 6.5-8.5.
Disclaimer:	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Purity	≥98% (HPLC)
mp	212 °C
Storage Temp.	−20°C
UNSPSC	12352203

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