Monoclonal Anti-Heat Shock Protein 90 antibody produced in mouse
SIGMA/H1775 - clone AC-16, ascites fluid
Synonym: Anti-EL52; Anti-HEL-S-65p; Anti-HSP86; Anti-HSP89A; Anti-HSP90A; Anti-HSP90N; Anti-HSPC1; Anti-HSPCA; Anti-HSPCAL1; Anti-HSPCAL4; Anti-HSPN; Anti-Hsp103; Anti-Hsp89; Anti-Hsp90; Anti-LAP-2; Anti-LAP2
MDL Number: MFCD02189140
Product Type: Chemical
Western Blotting Whole extract of HS-68 Human foreskin fibroblast cells were separated on SDS-PAGE and probed with Monoclonal Anti-Heat Shock Protein 90 antibody produced in Mouse, Clone: AC-16 (Cat. No. H1775). The antibody was developed using Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A9917). Lanes 1. 1:1,000 antibody 2. Negative Control
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | AC-16, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG2b |
| mol wt | antigen 88 kDa |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | wheat germ, chicken, Sf9 cell line, rabbit, rat, human, mouse, Achlya |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | microarray: suitable |
| western blot: 1:1,000 using cultured human foreskin fibroblasts | |
| UniProt accession no. | P07900  |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Immunohistochemistry (1 paper) Western Blotting (1 paper) |
| Application: | Monoclonal Anti-Heat Shock Protein 90 antibody has been used in ovarian histomorphology, immunohistochemistry and western blotting. |
| Biochem/physiol Actions: | HSP90AB1 (heat shock protein 90α family class B member 1) participates in signal transduction, protein folding and degradation, and morphological evolution. It is required for the transport of client proteins between the cytoplasm and nucleus. In lung adenocarcinoma patients, overexpression of HSP90AB1 in non-small cell lung cancer tissues results in imperfect clinical predictions. Suppression of this gene reduces the potential of endothelial cells to form tube structures. |
| Biochem/physiol Actions: | The antibody is reactive with both the constitutive and the inducible forms of HSP90. However, it does not bind to the native form of HSP90. It does not recognize HSP90 from E. coli and yeast. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | HSP90AB1 (heat shock protein 90α family class B member 1) belongs to the Hsp90 family. It consists of major members-Hsp90α, Hsp90β, GRP94 (glucose-regulated protein 94) and Hsp75. It is located on chromosome 6p21. HSP90AB1is an ATP-dependent molecular chaperone, having a molecular weight of 90kDa. HSP90AA1 is located on human chromosome 14q32.31. |
| Immunogen: | heat shock protein 90 (HSP90) from the water mold Achlya ambisexualis |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |