Anti-Histone Deacetylase 7 (HDAC7) (KG-17) antibody produced in rabbit
SIGMA/H2662 - affinity isolated antibody, buffered aqueous solution
Synonym: Anti-HD7; Anti-HD7A; Anti-HDAC7A
MDL Number: MFCD04040364
Product Type: Chemical
Immunoprecipitation
HDAC7 was immunoprecipitated from whole cell extracts of human HEK 293T cells expressing HDAC7-FLAG using Anti-HDAC7 (KG-17) (Cat. No. H2662). The IP samples were separated on SDS-PAGE, blotted with ANTI-FLAG® (Cat No. F7425) and developed using Anti-Rabbit IgG, Peroxidase (Cat. No. A0545) and chemiluminescent substrate. (Lane 1) 1.8 μg antibody immunoprecipitates HDAC7 from a RIPA extract of 180 μg of 293T cells expressing HDAC7-FLAG; (Lane 2) negative control (extract without antibody).
HDAC7 was immunoprecipitated from whole cell extracts of human HEK 293T cells expressing HDAC7-FLAG using Anti-HDAC7 (KG-17) (Cat. No. H2662). The IP samples were separated on SDS-PAGE, blotted with ANTI-FLAG® (Cat No. F7425) and developed using Anti-Rabbit IgG, Peroxidase (Cat. No. A0545) and chemiluminescent substrate. (Lane 1) 1.8 μg antibody immunoprecipitates HDAC7 from a RIPA extract of 180 μg of 293T cells expressing HDAC7-FLAG; (Lane 2) negative control (extract without antibody).
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 1:500 Rabbit Anti-Histone Deacetylase 7 (HDAC7) (KG-17) (Cat. No. H2662). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. HeLa nuclear 2. NIH-3T3
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~105 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, human, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 1.0-1.5 μg using RIPA extract of 293T cells expressing recombinant human HDAC7 |
| indirect immunofluorescence: 1:50 using rat NRK cells | |
| western blot: 1:1,000 using whole extracts of mouse NIH-3T3 cells | |
| UniProt accession no. | Q8WUI4  |
| Application: | Anti-Histone Deacetylase 7 (HDAC7) (KG-17) antibody produced in rabbit has been used in • indirect immunofluorescene • immunoblotting • immunoprecipitation |
| Biochem/physiol Actions: | Anti-Histone Deacetylase 7 recognizes human, mouse, and rat HDAC7. |
| Biochem/physiol Actions: | Class II histone deacetylases (HDACs) have been implicated in the regulation of muscle differentiation. Interaction of HDAC4, -5, and -7 with members of the myocyte enhancer factor-2 (Mef2) family of transcription factors represses their transcriptional activity and prevents myogenesis. Shuttling of HDAC7 between the cell nucleus and the cytoplasm is controlled by a mechanism involving calmodulin independent kinase I (CaMKI) and 14-3-3 proteins. The HDAC7 enzymatic activity depends on its interaction with the class I HDAC3, and the corepressors silencing mediator of retinoic acid and thyroid hormone receptors (SMRT) and nuclear receptor co-repressor (N-COR). HDAC7 also interacts with the transcriptional repressor B-cell lymphoma 6 (BCL-6). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Mammalian histone deacetylases (HDACs) can be divided into three classes according to sequence homology. Class I consists of the yeast transcriptional regulatory protein Rpd3-like proteins HDAC1, HDAC2, HDAC3, and HDAC8. Class II consists of the yeast Hda1-like proteins HDAC4, HDAC5, HDAC6, HDAC7, HDAC9 and HDAC10. Class III consists of the yeast sirtuin 2 (Sir2)-like proteins. Although HDAC7 is localized mostly to the cell nucleus, it is also found in the cytoplasm. |
| Immunogen: | Synthetic peptide corresponding to amino acids of human HDAC7, conjugated to KLH. |
| Packaging: | 200 μL in glass insert |
| Physical form: | Solution in 0.01 M phosphate buffered saline containing 1% bovine serum albumin and 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |