Anti-Histone Deacetylase 2 (HDAC2) antibody, Mouse monoclonal
SIGMA/H2663 - clone HDAC2-62, purified from hybridoma cell culture
MDL Number: MFCD02096544
Product Type: Chemical
Western Blotting JOURNAL CITATION: Delaying histone deacetylase response to injury accelerates conversion into repair Schwann cells and nerve regeneration. By: Brügger, V., Duman, M., et al. in Nat Commun, 2017. PubMed ID: 28139683 Image collected and cropped by CiteAb from the following publication, (http://www.nature.com/doifinder/10.1038/ncomms14272), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Western Blotting JOURNAL CITATION: Delaying histone deacetylase response to injury accelerates conversion into repair Schwann cells and nerve regeneration. By: Brügger, V., Duman, M., et al. in Nat Commun, 2017. PubMed ID: 28139683 Image collected and cropped by CiteAb from the following publication, (http://www.nature.com/doifinder/10.1038/ncomms14272), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunofluorescence HeLa cells were fixed and permeabilized with cold methanol followed by cold methanol:acetone Fixed cells were stained with 10 μg/mL Monoclonal Anti-Histone Deacetylase 2 Clone: HDAC2-62 (Cat. No. H2663). The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate.
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 0.5 μg/mL Monoclonal Anti-Histone Deacetylase 2 Clone: HDAC2-62 (Cat. No. H2663). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. HEK-293T 2. HeLa 3. JURKAT 4. K562 5. Neuro-2a 6. NIH-3T3
Immunoprecipitation Monoclonal Anti-HDAC2 Clone: HDAC2-62 (Cat. No. H2663) was used to immunoprecipitate HDAC2 from HeLa whole cells extract. Detection antibody: Rabbit Anti-HDAC2 (Cat. No. H3159). Lanes 1. 5 μg antibody 2. 2.5 μg antibody 3. Negative control: 5 μg/mL non-relevant antibody
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | HDAC2-62, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2b |
| mol wt | antigen ~55 kDa |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, chicken, bovine, canine, rat, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: suitable |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: suitable | |
| microarray: suitable | |
| western blot: 0.25-0.5 μg/mL using total cell extracts from NIH3T3 cells | |
| UniProt accession no. | Q92769  |
| Application: | Anti-Histone Deacetylase 2 (HDAC2) antibody, Mouse monoclonal has been used in: • co-immunoprecipitation • chromatin immunoprecipitation (ChIP) • western blot • immunohistochemistry • enzyme linked immunosorbent assays (ELISA) |
| Biochem/physiol Actions: | Histone Deacetylases including HDAC′s 1-10 facilitate transcriptional repression and nucleosome condensation. Abnormal recruitment of HDACs by various oncoproteins may occur in certain neoplastic diseases. Inhibition of HDAC2 activity by valproic acid induces proteasomal degradation of HDAC2. |
| Biochem/physiol Actions: | Monoclonal Anti-Histone Deacetylase 2 (HDAC2) recognizes human, bovine, dog, rat, mouse, and chicken HDAC2 (approx. 55 kDa). The antibody epitope resides within amino acids 471-488 of human HDAC2. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Anti-Histone Deacetylase 2 (HDAC2) antibody, Mouse monoclonal (mouse IgG2b isotype) is derived from the HDAC2-62 hybridoma produced by the fusion of mouse myeloma cells (NS1) and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to the C-terminal region of human histone deacetylase 2 (amino acid residues with N-terminal added cysteine) conjugated to maleimide-activated KLH. Histone deacetylase 2 (HDAC2) is the catalytic subunits of different multiprotein regulatory complexes. |
| Packaging: | 200 μL in glass insert |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |