Anti-Hyperpolarization-Activated Cation Channel HCN2 antibody produced in rabbit
SIGMA/H2769 - affinity isolated antibody, lyophilized powder
MDL Number: MFCD02686271
Product Type: Chemical
Immunohistochemistry Anti-HCN2: Cat. No. H2769: Immunochistochemical staining of Rat cerebellum frozen sections using Anti-HCN2 antibody. A. HCN2 (red) appears in Purkinje cells (arrows). B. Staining of astrocytes with Mouse Anti-Glial fibrillary acidic protein (GFAP, green demonstrates the restriction of HCN2 to neuronal cell bodies. C. Confocal merge of HCN2 and GFAP images demonstrates the respective localization of these proteins.
Immunohistochemistry Anti-HCN2: Cat. No. H2769: Immunohistochemical staining of Mouse hypothalamus using Anti-HCN2 antibody. A. HCN2 (red) appears in cells of the paraventricular nucleus (PVN, arrows). B. Staining of paraventricular nerve cells with Mouse Anti-Calcium binding protein (CBD28k, green). C. Confocal merge of HCN2 and CBD28k demonstrates some co-localization. V = Third ventricle.
Immunohistochemistry Anti-HCN2: Cat. No. H2769: Immunohistochemical staining of Mouse thalamus frozen section using Guinea Pig Anti-HCN4 antibody and Rabbit Anti-HCN2 antibody. A. Staining of HCN4 (green) appears in the ventral posterior thalamic nucleus (VPL). B. In the same section as in A, staining of HCN2 (red) appears in the ventral posterior thalamic nucleus (VPL) and also in the reticular thalamic nucleus (RT). The area between these thalamic nuclei (star) is white matter and neither protein is expressed in that region. C. Merged images of A and B.
Immunocytochemistry Anti-HCN2: Cat. No. H2769: Immunocytochemical staining of paraformaldehyde-fixed and permeabilized Rat dorsal root ganglion (DRG) primary culture using Anti-HCN2 antibody, (1:100), (green). Cells were stained with Anti-HCN2 antibody followed by Goat Anti-Rabbit-AlexaFluor-488 secondary antibody. Nuclear staining of cells using the cell-permeable DNA dye Hoechst 33342 (blue).
Immunoblotting Anti-HCN2: Cat. No. H2769: Western blot analysis of Rat brain membranes. Lanes 1. Anti-HCN2 antibody, (1:200). 2. Anti-HCN2 antibody, preincubated with the control peptide antigen.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | lyophilized powder |
| Quality Level | 100  |
| species reactivity | mouse, human, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| immunohistochemistry: suitable | |
| immunoprecipitation (IP): suitable | |
| western blot: 1:200-1:1000 using Rat brain membranes | |
| UniProt accession no. | Q9UL51  |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | synthetic peptide corresponding to amino acids 147-161 of human HCN2 (with additional N-terminal cysteine). The epitope is identical in mouse. |
| Physical form: | Lyophilized at 0.3 mg/mL from Phosphate Buffered Saline, pH 7.4, containing 1% Bovine Serum Albumin, and 0.05% Sodium Azide |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |