Anti-Histone Deacetylase 3 (HDAC3) antibody produced in rabbit
SIGMA/H3034 - IgG fraction of antiserum, buffered aqueous solution
Synonym: Anti-HD3
MDL Number: MFCD02096545
Product Type: Chemical
Immunohistochemistry Formalin-fixed, paraffin-embedded section of lymph node stained with 1:500 Anti-Histone Deacetylase 3 (HDAC3) antibody produced in Rabbit (Cat. No. H3034) followed by Anti-Rabbit IgG (whole molecule)-Biotin antibody produced in Goat (Cat. No. B8895) and by ExtrAvidin®-Peroxidase (Cat. No. E2886).
Immunofluorescence HeLa cells were fixed and permeabilized with cold methanol followed by cold methanol:acetone. Fixed cells were stained with 1:200 Rabbit Anti-HDAC3 (Cat. No. H3034). The antibody was developed using Goat Anti-Rabbit IgG, Cy3 conjugate.
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 1:5,000 Rabbit Anti-HDAC3 (Cat. No. H3034). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. HEK-293T; 2. HeLa; 3. K562; 4. COS-7; 5. CHO; 6. NIH-3T3; 7. Neuro-2a; 8. PC-12; 9. NRK
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 50 kDa |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rat, mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using human lymph node sections |
| immunoprecipitation (IP): 1:2,500 using whole lysate of NIH3T3 cells | |
| microarray: suitable | |
| western blot: 1:10,000 using nuclear extract from HeLa human epithelioid carcinoma cells | |
| western blot: 1:2,000 using whole extract of PC-12 rat pheochromocytoma cell line | |
| UniProt accession no. | O15379  |
| Application: | Anti-Histone Deacetylase 3 (HDAC3) antibody produced in rabbit has been used in: • immunohistochemistry • western blotting • immunoprecipitation |
| Biochem/physiol Actions: | Anti-Histone Deacetylase 3 specifically recognises histone deacetylase 3 by immunoblotting and immunoprecipitation (50 kDa). An additional band of lower molecular weight may be detected in some cell line extracts by immunoblotting. Staining of HDAC3 by immunoblotting is specifically inhibited with the immunizing peptide. The product is also useful for the detection of HDAC3 by immunohistochemistry. The epitope(s) recognized by the antibody is resistant to routine formalin-fixation and paraffin-embedding. The antibody reacts with HDAC3 of human, rat, and mouse origin. |
| Biochem/physiol Actions: | Histone acetylation is a dynamic process whose levels are determined by the net activities of HATs and the competing enzymes histone deacetylases (HDACs). Both activities are associated with the nuclear matrix. Six or seven different mammalian HDACs have been described. HDAC1, HDAC2 and HDAC3 are similar to yeast Rpd3 protein, while HDAC4, HDAC5 and HDAC6 are similar to yeast Hda1 protein. Histone deacetylases activities were often, but not always, associated with transcriptional repression and nucleosomal condensations. HDAC1, HDAC2 and several other HDACs are the catalytic subunits of different multiprotein regulatory complexes. |
| Biochem/physiol Actions: | Histone deacetylase 3 (HDAC3) acts as a transcriptional modulator of inflammatory genes. It deacetylates lysine residues of histones. HDAC3 associates with nuclear receptor co-repressor (NCoR) or silencing mediator of retinoic and thyroid receptors (SMRTs) and activates the complex. This protein participates in interleukin-1 expression and inflammatory responses. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Histone deacetylase 3 (HDAC3) is an epigenetic modifier and is expressed in various tissues. It belongs to the class I subfamily of histone deacetylases. The HDAC3 gene is mapped on to human chromosome 5q31.3. |
| Immunogen: | synthetic peptide corresponding to amino acids 411-428 of human and chicken HDAC3. |
| Physical form: | Solution in 0.01 M phosphate bufffered saline, pH 7.4, containing 15 mM sodium azide. |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |