Anti-Histone Deacetylase 2 (HDAC2) antibody produced in rabbit
SIGMA/H3159 - IgG fraction of antiserum, buffered aqueous solution
Synonym: Anti-HD2; Anti-KDAC2; Anti-RPD3; Anti-YAF1
MDL Number: MFCD02096544
Product Type: Chemical
Immunohistochemistry Formalin fixed, paraffin-embedded Rat lymph node tissue sections were stained with 1:500 Anti-Histone Deacetylase 2 (HDAC2) antibody produced in Rabbit (Cat. No. H3159) followed by 20 μg/mL Anti-Rabbit IgG (H+L)-Biotin antibody produced in Goat and by 20 μg/mL ExtrAvidin®-Peroxidase.
Immunoprecipitation Rabbit Anti-HDAC2 (Cat. No. H3159) was used to immunoprecipitate HDAC2 from HeLa whole cell extract. Detection antibody: Rabbit Anti-HDAC2 (Cat. No. H3159). Lanes 1. 5 μL antibody 2. 10 μL antibody 3. Negative control: No extract
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 55 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using protease-digested, human lymph node sections |
| immunoprecipitation (IP): 50 μg using whole lysate of NIH3T3 cells | |
| microarray: suitable | |
| western blot: 1:20,000 using nuclear extract from human HeLa cells | |
| UniProt accession no. | Q92769  |
| Application: | Anti-Histone Deacetylase 2 (HDAC2) antibody has been used: • in immunoblotting and dot blot • in immunoprecipitation • in immunohistochemistry • in enzyme linked immunosorbent assay (ELISA) • in western blotting • in chromatin immunoprecipitation(ChiP) |
| Biochem/physiol Actions: | Anti-Histone Deacetylase 2 specifically recognizes histone deacetylase 2 by immunoblotting and immunoprecipitation (55 kDa). An additional band of lower molecular weight may be detected in some cell line extracts by immunoblotting. Staining of HDAC2 by immunoblotting is specifically inhibited with the immunizing peptide. The product is also useful for the detection of HDAC2 by immunohistochemistry. The epitope(s) recognized by the antibody is resistant to routine formalin-fixation and paraffin embedding. The antibody reacts with HDAC2 of human, rat and mouse origin. |
| Biochem/physiol Actions: | Histone acetylation is a dynamic process whose levels are determined by the net activities of Histone acetyl transferase (HATs) and the competing enzymes histone deacetylases (HDACs). Both activities are associated with the nuclear matrix. Histone deacetylases activities were often, but not always, associated with transcriptional repression and nucleosome condensations. HDAC1, HDAC2 and several other HDACs are the catalytic subunits of different multiprotein regulatory complexes. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Histone Deacetylase (HDACs) have been described as six or seven different groups in mammals. HDAC1, HDAC2 and HDAC3 are like yeast Rpd3 protein, while HDAC4, HDAC5 and HDAC6 are like yeast Hda1 (Histone Deacetylase 1) protein. |
| Immunogen: | synthetic peptide (C-SGEKTDTKGTKSEQLSNP) corresponding to the C-terminus region of histone deacetylase 2 of human origin (amino acids residues 471-488 with N-ternimal added cysteine) conjugated to maleimide-activated KLH. This sequence is highly conserved in mouse and chicken (2 amino acids and 1 amino acid substitutions, respectively). |
| Physical form: | Solution in 0.01 M phosphate bufffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |