Hygromycin B from Streptomyces hygroscopicus
SIGMA/H3274 - powder, BioReagent, suitable for cell culture, suitable for insect cell culture
Synonym: Hygromycin
CAS Number: 31282-04-9
Empirical Formula (Hill Notation): C20H37N3O13
Molecular Weight: 527.52
EC Number: 250-545-5
MDL Number: MFCD06795479
Linear Formula: C20H37N3O13
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: H3274-100MG
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: H3274-250MG
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: H3274-50MG
| (1) 7.1, (2) 8.8 | |
| antibiotic activity spectrum | fungi |
| biological source | Streptomyces hygroscopicus |
| color | white to beige |
| faintly brown to brown | |
| concentration | ≥60% (HPLC) |
| form | powder |
| InChI | 1S/C20H37N3O13/c1-23-7-2- |
| InChI key | GRRNUXAQVGOGFE-XKIAHZFYSA |
| mode of action | protein synthesis | interferes |
| mp | 160-180 °C |
| product line | BioReagent |
| Quality Level | 200  |
| SMILES string | CN[C@H]1C[C@@H](N)[C@H](O |
| solubility | H2O: 50 mg/mL (As a stock solution. Stock solutions should be stored at 2-8°C. Stable at 37°C for 30 days.) |
| storage temp. | 2-8°C |
| technique(s) | cell culture | insect: suitable |
| cell culture | mammalian: suitable |
| Application: | Hygromycin B antibiotic was used for hygro resistance effect in the following cells: • transfected 293T cells with mixture of plasmids at 160 μg/ml. • MCF-7ALOX12 cells at 150 μg/ml. • MSCV Luciferase PGK-hygro plasmid. • STAT3 Reporter HeLa Stable Cell Line (stably transfected with Luciferase gene under the control of STAT3 promoter) maintained in DMEM supplemented with 10% FBS. |
| Application: | Hygromycin B is an aminoglycoside antibiotic isolated from Streptomyces hygroscopicus. It has been used to study protein synthesis at the level of the 70S ribosome translocation and mRNA template misreading, as an antiviral agent by selectively penetrating cells rendered permeable by virus infection and inhibiting translation, and as a selection agent for hygromycin resistance gene transformed cells. It is recommended for use as a selection agent at 100-800 μg/mL, specifically at 100 μg/mL for prokaryotes, 200 μg/mL for lower eukaryotes and 150-400 μg/mL for higher eukaryotes. |
| Biochem/physiol Actions: | Mode of Action: The product acts by inhibiting protein synthesis by inducing the misreading of the m-RNA template in the prokaryote, with the potency to inhibit translation. Antimicrobial Spectrum: Hygromycin B acts against bacteria, fungi and higher eukaryotic cells. |
| Disclaimer: | Hygromycin B products should be stored as supplied at 2-8°C, and the dry solid is table for at least 5 years if stored at 2-8°C. It is stable at 37°C for 30 days. (For solutions) This solution is stable as supplied for two years if stored at 2-8°C. |
| General description: | Aminoglycosides are the most widely used class of antibiotics that target ribosome. Hygromycin B is an aminocyclitol antibiotic. The organism, S. hygroscopicus contains phosphotransferase (HPH) activity, which phosphorylates hygromycin B helping in autoimmunity against the toxic effects of the drug. |
| General description: | Chemical structure: aminoglycoside |
| Preparation Note: | This product is purified by ion exchange chromatography. Hygromycin B is soluble in water at concentrations >50 mg/mL, and also soluble in methanol or ethanol. Solutions should be sterilized by filtration, not by autoclaving. Additionally, Hygromycin B solutions have been reported to lose activity on freezing, and since they are stable refrigerated, freezing should be avoided. |
| Symbol |  GHS06 |
| Signal word | Danger |
| Hazard statements | H300 + H310 + H330 |
| Precautionary statements | P260 - P262 - P264 - P280 - P302 + P352 + P310 - P304 + P340 + P310 |
| Hazard Codes | T+ |
| Risk Statements | 26/27/28-41-42/43 |
| Safety Statements | 22-26-28-36/37/39-45 |
| RIDADR | UN3462 - class 6.1 - PG 1 - Toxins, extracted from living source |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| mp | 160-180 °C |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352207 |