Anti-Histone Deacetylase 1 (HDAC1) antibody produced in rabbit
SIGMA/H3284 - IgG fraction of antiserum, buffered aqueous solution
Synonym: Anti-GON-10; Anti-HD1; Anti-KDAC1; Anti-RPD3; Anti-RPD3L1
MDL Number: MFCD01633420
Product Type: Chemical
Immunocytochemistry-immunoflourescence JOURNAL CITATION: NF-Y dependent epigenetic modifications discriminate between proliferating and postmitotic tissue. By: Gurtner, A., Fuschi, P., et al. in PLoS One, 2008. PubMed ID: 18431504 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0002047), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunohistochemistry formalin fixed, paraffin-embedded Human lymph node tissue sections were stained with 1:500 Anti-Histone Deacetylase 1 (HDAC1) antibody produced in Rabbit (Cat. No. H3284) followed 20 μg/mL Goat Anti-Rabbit IgG (whole molecule)-Biotin (Cat. No. B8895) followed by 20 μg/mL ExtrAvidin®-Peroxidase (Cat. No. E2886)
Immunofluorescence HeLa cells were fixed and permeabilized with cold methanol followed by cold methanol:acetone. Fixed cells were stained with 1:200 Rabbit Anti-HDAC1 (Cat. No. H3284). The antibody was developed using Goat Anti-Rabbit IgG, Cy3 conjugate.
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 1:10,000 Rabbit Anti-HDAC1 (Cat. No. H3284). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. HEK-293T; 2. HeLa; 3. K562; 4. CHO; 5. NIH-3T3; 6. PC-12
Immunoprecipitation Rabbit Anti-HDAC1 (Cat. No. H3284) was used to immunoprecipitate HDAC1 from HeLa whole cell extract. Detection antibody: Anti-HDAC1 (Cat. No. H3284). Lanes 1. 5 μL antibody 2. 10 μL antibody 3. Negative control: 10 μL non-relevant antibody
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 65 kDa |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using human lymph node sections |
| immunoprecipitation (IP): 5-10 μL using whole lysate of NIH3T3 cells | |
| microarray: suitable | |
| western blot: 1:20,000 using nuclear extract from HeLa human epithelioid carcinoma cells | |
| UniProt accession no. | Q13547  |
| Application: | Anti-Histone Deacetylase 1 (HDAC1) antibody can be used in immunoblotting; immunoprecipitation and immunohistochemistry. It is also useful in chromatin immunoprecipitation. |
| Application: | Anti-Histone Deacetylase 1 (HDAC1) antibody has been used in immunohistochemical staining and chromatin immunoprecipitation (ChIP) analyses. |
| Application: | Anti-Histone Deacetylase 1 is suitable for immunoblotting; immunoprecipitation and immunohistochemistry. (IHC) Immunoblotting: a minimum working dilution of 1:20,000 is determined using a nuclear extract of HeLa human epithelioid carcinoma cell line. Immunoblotting: a minimum working dilution of 1:2,000 is determined using a whole extract of PC-12 rat pheochromocytoma cell line. Immunoprecipitation: a recommended working volume of 5-10 ml is determined using a whole lysate of NIH 3T3 cells. Indirect immunoperoxidase staining: a minimum working dilution of 1:500 is determined using protease-digested, formalin-fixed, paraffin-embedded human lymph node sections. |
| Biochem/physiol Actions: | Anti-Histone Deacetylase 1 specifically recognizes histone deacetylase 1 by immunoblotting and immunoprecipitation (65 kDa). An additional band of lower molecular weight may be detected in some cell line extracts by immunoblotting. Staining of HDAC1 by immunoblotting is specifically inhibited with the immunizing peptide. The antibody is also useful for the detection of HDAC1 by immunohistochemistry. The epitope(s) recognized by the antibody is resistant to routine formalin-fixation and paraffin-embedding, unless there is a protease digestion. The antibody reacts with HDAC1 of human, rat and mouse origin. |
| Biochem/physiol Actions: | Histone deacetylase 1 (HDAC1) prevents the infection of influenza A virus (IAV). The protein plays important roles in DNA repair, splicing, regulation of gene expression and cell division. Overexpression of this gene can be seen in cancers like breast, renal cell cancer, and classical Hodgkin′s lymphoma. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Acetylation is a histone modification in which epsilon amino group of conserve lysine residue is modified with enzymes known as histone acetyltransferases (HATs). HATs are present in two forms nuclear and cytoplasmic. Histone deacetylases are competating enzymes of HATs and are often associated with transcriptional repression and nucleosome condensation. Anti-Histone Deacetylase 1 (HDAC1) antibody can be used in chromatin immunoprecipitation. . This antibody can also be used in double immunofloresence labeling. Anti-Histone Deacetylase 1 (HDAC1) antibody specifically recognizes histone deacetylase 1and reacts specifically with HDAC1 of human, rat and mouse. |
| General description: | Histone acetylation is a dynamic process whose levels are determined by the net activities of HATs and the competing enzymes histone deacetylases (HDACs). Both activities are associated with the nuclear matrix. Six or seven different mammalian HDACs have been described. HDAC1, HDAC2 and HDAC3 are similar to yeast Rpd3 protein, while HDAC4, HDAC5 and HDAC6 are similar to yeast Hda1 protein. Histone deacetylases activities were often, but not always, associated with transcriptional repression and nucleosomal condensations. HDAC1, HDAC2 and several other HDACs are the catalytic subunits of different multiprotein regulatory complexes. |
| General description: | Histone deacetylase 1 (HDAC1) is located in the nucleus. It belongs to class I of histone deacetylases. HDAC1 is located on human chromosome 1p35. |
| Immunogen: | synthetic peptide corresponding to amino acids 466-482 of human and mouse HDAC1. |
| Physical form: | Solution in 0.01 M phosphate bufffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |