Anti-Histone Deacetylase 10 (HDAC10) antibody produced in rabbit
SIGMA/H3413 - affinity isolated antibody, buffered aqueous solution
Synonym: Anti-HD10
MDL Number: MFCD06201937
Product Type: Chemical
Immunoblotting
Mouse NIH-3T3 cell whole extracts were separated on SDS-PAGE, blotted with 0.8 μg/mL Anti-HDAC10 (Cat. No. H3413) and developed using Anti-Rabbit IgG, Peroxidase (Cat. No. A0545) and a chemiluminescent substrate.
Mouse NIH-3T3 cell whole extracts were separated on SDS-PAGE, blotted with 0.8 μg/mL Anti-HDAC10 (Cat. No. H3413) and developed using Anti-Rabbit IgG, Peroxidase (Cat. No. A0545) and a chemiluminescent substrate.
Immunofluorescence HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 10 μg/mL Anti-Histone Deacetylase 10 (HDAC10) antibody produced in Rabbit (Cat. No. H3413) followed by 1:200 Anti-Rabbit IgG (whole molecule)-FITC antibody produced in Goat (Cat. No. F9887).
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 1 μg/mL Rabbit Anti-Histone Deacetylase 10 (HDAC10) (Cat. No. H3413). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. K562 2. NIH-3T3 3. Neuro-2a 4. PC-12 5. NRK
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~72 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, rat, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect immunofluorescence: 10-20 μg/mL using human HeLa cells |
| microarray: suitable | |
| western blot (chemiluminescent): 0.5-1.0 μg/mL using whole extracts of mouse NIH3T3 and rat NRK cells | |
| UniProt accession no. | Q969S8  |
| Application: | Anti-Histone Deacetylase 10 (HDAC10) antibody produced in rabbit has been used in western blot analysis and immunofluorescence. |
| Biochem/physiol Actions: | Histone deacetylase 10 (HDAC10) can deacetylate histones, repress transcription and interact with HDAC3. HDAC10 can stimulate lung cancer proliferation via AKT phosphorylation. It is involved in homologous recombination. |
| Biochem/physiol Actions: | The leucine-rich domain on the C-terminal of HDAC8 is responsible for cytoplasmic enrichment. Trichostatin A (TSA), a specific antitumor deacetylase inhibitor is sensitive to the enzymatic activity of HDAC10. HDAC10 attaches to a promoter and represses transcription in the nucleas. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Histone deacetylase 10 (HDAC10) is highly expressed in liver, kidney, pancreas and spleen. HDAC10 is similar to HDAC6, both containing a unique putative second catalytic domain not found in other HDACs. HDAC10 is localized to both the nucleus and cytoplasm. Histone deacetylases (HDACs) are competing enzymes, belonging to histone deacetylase family. HDAC10 is a novel member of the class II family of HDACs with a bipartite structure consisting of Hda1p-related catalytic domain at the N-terminal and a leucine-rich domain at the C-terminal. It is present, both in the nucleas and cytoplasm. |
| Immunogen: | synthetic peptide corresponding to amino acid residues 2-16 of human HDAC10 with C-terminal added cysteine, conjugated to KLH. |
| Packaging: | 200 μL in glass bottle |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |