Monoclonal Anti-Heat Shock Protein 60 antibody produced in mouse
SIGMA/H3524 - clone LK2, ascites fluid
Synonym: Anti-CPN60; Anti-GROEL; Anti-HLD4; Anti-HSP-60; Anti-HSP60; Anti-HSP65; Anti-HuCHA60; Anti-SPG13
MDL Number: MFCD02189138
Product Type: Chemical
Western Blotting Whole extract of Human forskin fibroblasts HS-68 cells were separated on SDS-PAGE and probed with Monoclonal Anti-Heat Shock Protein 60 antibody produced in Mouse, Clone: LK2 (Cat. No. H3524). The antibody was developed using 1:500 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A9917). Lanes 1. 1:100 antibody 2. 1:200 antibody 3. Negative Control
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | LK2, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG1 |
| mol wt | antigen 60 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | spinach, chicken, E. coli, helminth, rat, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | electron microscopy: suitable |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| indirect ELISA: suitable | |
| western blot: 1:100 using cultured human foreskin fibroblast extract | |
| UniProt accession no. | P10809  |
| Application: | Monoclonal Anti-Heat Shock Protein 60 antibody produced in mouse has been used in:western blotting, immunocytochemistry, enzyme linked immuno sorbent assay (ELISA) ,immunoblotting |
| Biochem/physiol Actions: | A wide variety of environmental perturbations, such as a sudden increase in temperature, induce cells to rapidly synthesize a group of polypeptides known as the heat shock (stress) proteins. The 60 kDa HSP family (HSP60) which retained a uniquely high level of sequence conservation during evolution is a focus of interest as a potential antigen in a number of autoimmune diseases. Abnormal immune reactivity involving HSP60 has also been implicated in the pathogenesis of schizophrenia. In human arthritis and experimentally-induced arthritis in animals, disease development was seen to coincide with development of immune reactivity directed against not only bacterial HSP60, but also against its mammalian homologue. A human mitochondrial protein, originally designated P1, has been described as the human homologue of the bacterial HSP60, and >45% of the protein has sequence identity with its bacterial homologue (groEL, HSP65). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Heat Shock Protein 60 (HSP60), clone LK2, recognizes an epitope located between amino acid residues 383-419 of the human (corresponding to a.a. residues 356-393 of the mycobacterial) HSP60. The antibody is reactive against the mammalian (e.g., human, rat), avian (e.g., chicken), bacterial (e.g., E. coli), helminths and spinach HSP60. In immunoblotting, the antibody may label additional bands at approx. 38 and 97 kDa. It is reactive with both the constitutive and the inducible HSP60. It shows a raised level of staining in immunohistochemistry of formalin-fixed, paraffin-embedded synovial membranes taken from patients with juvenile chronic arthritis. |
| General description: | Monoclonal anti-Heat Shock Protein 60 (mouse IgG1 isotype) is derived from the LK2 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with recombinant human heat shock protein 60. |
| Immunogen: | recombinant human heat shock protein 60 (HSP60). |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |