HEPES solution
SIGMA/H3537 - BioPerformance Certified, 1 M, suitable for cell culture, 0.2 μm filtered
Synonym: N-
CAS Number: 7365-45-9
MDL Number: MFCD00006158
Product Type: Chemical
| application(s) | diagnostic assay manufacturing |
| cation traces | Fe: <5 ppm |
| heavy metals (as Pb): <5 ppm | |
| concentration | 1 M |
| form | liquid |
| grade | BioPerformance Certified |
| impurities | Bioburden, tested |
| DNase, RNase, Protease, Nickase, free | |
| endotoxin, tested | |
| InChI | 1S/C8H18N2O4S/c11-7-5-9-1 |
| InChI key | JKMHFZQWWAIEOD-UHFFFAOYSA |
| pH | 5.0-6.0 |
| Quality Level | 200  |
| SMILES string | OCCN1CCN(CCS(=O)(O)=O)CC1 |
| sterility | 0.2 μm filtered |
| suitability | suitable for molecular biology |
| technique(s) | cell culture | mammalian: suitable |
| useful pH range | 6.8-8.2 |
| Application: | RNAse HEPES has been used: • To supplement Dulbecco’smodified Eagle’smedium for maintenance of cell line and RPMI medium to wash rat islets • To recover purified ribonucleotide • As a component of HEPES/KOH buffer and adenylation buffer for small RNA isolation and sequencing • As a component in buffers used for nuclear extract preparation and also to supplement RPMI-1640 medium for maintenance of islets |
| General description: | HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| UNSPSC | 12161700 |