HEPES sodium salt solution
SIGMA/H3662 - BioReagent, 1M, suitable for cell culture
CAS Number: 75277-39-3
Empirical Formula (Hill Notation): C8H17N2NaO4S
Molecular Weight: 260.29
MDL Number: MFCD00036463
Linear Formula: C8H17N2NaO4S
Product Type: Chemical
| cation traces | Fe: <5 ppm |
| concentration | 0.90-1.10 M |
| form | solution |
| impurities | Bioburden, tested |
| DNase, RNase. Protease, Nickase, free | |
| endotoxin, tested | |
| <5 ppm Heavy metals (as Pb) | |
| InChI | 1S/C8H18N2O4S.Na/c11-7-5- |
| InChI key | RDZTWEVXRGYCFV-UHFFFAOYSA |
| product line | BioReagent |
| Quality Level | 200 ![]() |
| SMILES string | [Na+].OCCN1CCN(CC1)CCS([O |
| sterility | sterile-filtered (0.2 μm filtered) |
| technique(s) | cell culture | mammalian: suitable |
| Application: | HEPES sodium salt solution is suitable for use in cell culture studies. It may be used in the preparation of buffer solution of HEPES. It may be used as buffer during phosphorylation assays in permeabilized fibroblasts. It may be used to compose a complex dialysis buffer, used in the determination of free thyroxin in serum by radioimmunoassay. |
| General description: | HEPES is a general-purpose zwitterionic buffer which does not bind magnesium, calcium, manganese(II) or copper(II) ions. HEPES has been classified as Good′s buffer by Dr. Norman Good and his colleagues in 1966. It is zwitterionic at biological pH and is most effective in the pH range of pH 6.8 to 8.2. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| UNSPSC | 12161700 |

