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HEPES sodium salt solution

SIGMA/H3662 - BioReagent, 1M, suitable for cell culture

CAS Number: 75277-39-3
Empirical Formula (Hill Notation): C8H17N2NaO4S
Molecular Weight: 260.29
MDL Number: MFCD00036463
Linear Formula: C8H17N2NaO4S
Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-H3662-100ML 100 mL
$90.30
1/EA
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45-H3662-500ML 500 mL
$295.00
1/EA
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45-H3662-1L 1 L
$545.00
1/EA
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cation traces Fe: <5 ppm
concentration 0.90-1.10 M
form solution
impurities Bioburden, tested
  DNase, RNase. Protease, Nickase, free
  endotoxin, tested
  <5 ppm Heavy metals (as Pb)
InChI 1S/C8H18N2O4S.Na/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14;/h11H,1-8H2,(H,12,13,14);/q;+1/p-1
InChI key RDZTWEVXRGYCFV-UHFFFAOYSA-M
product line BioReagent
Quality Level 200 
SMILES string [Na+].OCCN1CCN(CC1)CCS([O-])(=O)=O
sterility sterile-filtered (0.2 μm filtered)
technique(s) cell culture | mammalian: suitable
Application: HEPES sodium salt solution is suitable for use in cell culture studies. It may be used in the preparation of buffer solution of HEPES. It may be used as buffer during phosphorylation assays in permeabilized fibroblasts. It may be used to compose a complex dialysis buffer, used in the determination of free thyroxin in serum by radioimmunoassay.
General description: HEPES is a general-purpose zwitterionic buffer which does not bind magnesium, calcium, manganese(II) or copper(II) ions. HEPES has been classified as Good′s buffer by Dr. Norman Good and his colleagues in 1966. It is zwitterionic at biological pH and is most effective in the pH range of pH 6.8 to 8.2.
RIDADR NONH for all modes of transport
WGK Germany WGK 3
Flash Point(F) Not applicable
Flash Point(C) Not applicable
UNSPSC 12161700

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