Anti-HA antibody, Mouse monoclonal
SIGMA/H3663 - clone HA-7, purified from hybridoma cell culture
Synonym: Monoclonal Anti-HA; Anti-HA; Anti-Influenza Hemagglutinin
MDL Number: MFCD00803873
Product Type: Chemical
Immunoblotting
Western blot analysis of a HA-LacZ titration: Decreasing amount of recombinant HA-LacZ were separated on SDS-PAGE and blotted with monoclonal Anti-HA, purified immunoglobulin (Cat. No. H3663) at a concentration of 1 μg/mL. The antibody was developed with A9917, Anti-Mouse IgG, Fab fragment, HRP conjugate, and a chemiluminescent substrate.
Western blot analysis of a HA-LacZ titration: Decreasing amount of recombinant HA-LacZ were separated on SDS-PAGE and blotted with monoclonal Anti-HA, purified immunoglobulin (Cat. No. H3663) at a concentration of 1 μg/mL. The antibody was developed with A9917, Anti-Mouse IgG, Fab fragment, HRP conjugate, and a chemiluminescent substrate.
Immunoblotting
Extract of HEK-293T cells transfected with HA-PAG608 (N terminal HA tag) was separated on SDS-PAGE and blotted with monoclonal Anti-HA, purified (Cat. No. H3663). The antibody was developed with Goat Anti-Mouse IgG, Peroxidase conjugate (Cat. No. A9917), and a chemiluminescent substrate. Each lane was incubated with decreasing antibody concentrations (2–0.125 μg/mL).
Extract of HEK-293T cells transfected with HA-PAG608 (N terminal HA tag) was separated on SDS-PAGE and blotted with monoclonal Anti-HA, purified (Cat. No. H3663). The antibody was developed with Goat Anti-Mouse IgG, Peroxidase conjugate (Cat. No. A9917), and a chemiluminescent substrate. Each lane was incubated with decreasing antibody concentrations (2–0.125 μg/mL).
Immunofluorescence HEK-293 cells overexpressing N-terminal HA tagged fusion protein were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton™ X-100. Fixed cells were stained with 1 μg/mL Monoclonal Anti-HA Clone: HA-7 (Cat. No. H3663). The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
Immunofluorescence HEK-293 cells overexpressing C-terminal HA tagged fusion protein were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton™ X-100. Fixed cells were stained with 1 μg/mL Monoclonal Anti-HA Clone: HA-7 (Cat. No. H3663). The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | HA-7, monoclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −20°C |
| technique(s) | immunocytochemistry: 1.0-2.0 μg/mL |
| immunoprecipitation (IP): 0.5-2.0 μg | |
| western blot: 0.25-1 μg/mL |
| Application: | Monoclonal Anti-HA antibody produced in mouse has been used: • in immunoblotting • in immunocytochemistry • in the immunoprecipitation |
| Biochem/physiol Actions: | Hemagglutinin (HA) binds to acid binding and is essential for the initiation of virus infection. It is the key target for the generation of vaccine. HA is the dominant antigen against which humoral immune response is provoked. Vaccines development involves the generation of antibody specific to the variable head and the conserved stalk region. Both the anti-stalk and anti-head antibodies may elicit protective function in influenza disease. |
| Biochem/physiol Actions: | The antibody recognizes native as well as denatured-reduced forms of HA-tagged proteins and is reactive with N- or C-terminal HA-tagged fusion proteins expressed in E. coli or in mammalian cells. |
| General description: | Hemagglutinin (HA) is a glycoprotein present in the membrane of influenza virus. HA exists as a trimer and each monomer at its membrane tip has receptor-binding site. Glycosylation event of HA occurs in endoplasmic reticulum. The head region with the receptor-binding site of the HA is variable. The mouse monoclonal Anti-HA, Clone HA-7 recognizes the human influenza virus hemagglutinin (HA), known as the HA tag. The product is reactive with HA-tagged fusion proteins expressed at either the amino or the carboxy terminus of the fusion protein. |
| Immunogen: | synthetic peptide corresponding to a fragment of human influenza virus hemagglutinin (HA) known as HA-tag, conjugated to KLH |
| Other Notes: | 2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research  |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide as a preservative. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |