Hyaluronidase from bovine testes
SIGMA/H3757 - Type VIII, lyophilized powder, 300-1,000 U/mg
Synonym: Hyaluronate 4-glycanohydrolase; Hyaluronoglucosaminidase
CAS Number: 37326-33-3
EC Number: 253-464-3
MDL Number: MFCD00131351
Product Type: Chemical
Hyaluronidase specificity: Hyaluronic acid is a polymer of alternating residues of β-D-(1-3) glucuronic acid and β-D-(1-4)-N-acetylglucosamine.
| biological source | bovine testis |
| conjugate | conjugate (Glucosaminoglycan) |
| form | lyophilized powder |
| mol wt | ~55 kDa (four subunits of 14 kDa each) |
| Quality Level | 200  |
| relevant disease(s) | cancer |
| specific activity | 300-1,000 U/mg |
| storage temp. | −20°C |
| type | Type VIII |
| UniProt accession no. | Q5E985  |
| Q8SQG8 |
| Biochem/physiol Actions: | Hyaluronidase degrades hyaluronan and has been found to be inappropriately regulated during cancer progression. |
| Biochem/physiol Actions: | These enzymes randomly cleave β-N-acetylhexosamine-[1→4 |
| Other Notes: | One unit will degrade 0.75 μg of the polysaccharide hyaluronic acid per minute at pH 5.35 at 37 ºC (as measured by turbidimetric absorbance (λ: 600nm) when complexed with BSA after 45 minutes). |
| Other Notes: | View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enz |
| Preparation Note: | Prepared from aseptic filled solution of Type I-S. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: 300-1,000 U/mg |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.2.1.35 ( BRENDA | IUBMB ) |
| UNSPSC | 12352204 |