HEPES sodium salt

SIGMA/H3784 - BioPerformance Certified, suitable for cell culture, ≥99.0%

Synonym: 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt; N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) sodium salt

CAS Number: 75277-39-3
Empirical Formula (Hill Notation): C₈H₁₇N₂NaO₄S
Molecular Weight: 260.29
EC Number: 278-169-7
MDL Number: MFCD00036463
Linear Formula: C₈H₁₇N₂NaO₄S
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-H3784-25G	25 g	$73.80 1/EA	Add To Favorites
45-H3784-100G	100 g	$195.00 1/EA	Add To Favorites
45-H3784-500G	500 g	$687.00 1/EA	Add To Favorites
45-H3784-1KG	1 kg	$1180.00 1/EA	Add To Favorites

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This image depicts SKU: H3784-25G

Properties
Descriptions
Safety Info.
Basic Data

absorption	≤0.02 at 280 in H₂O at 0.1 M
	≤0.04 at 260 in H₂O at 0.1 M
application(s)	cell analysis diagnostic assay manufacturing general analytical life science and biopharma
assay	≥99.0%
color	white
density	1.19 g/cm³ at 20—25 °C
	1.21 g/cm³ at 20—25 °C
foreign activity	DNase, RNase, NICKase, protease, none detected
form	powder
grade	BioPerformance Certified
impurities	endotoxin and total aerobic microbial count, tested
InChI	1S/C8H18N2O4S.Na/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14;/h11H,1-8H2,(H,12,13,14);/q;+1/p-1
InChI key	RDZTWEVXRGYCFV-UHFFFAOYSA-M
pH	10-12 (1 M in water)
pKa (25 °C)	7.5
Quality Level	400
SMILES string	[Na+].OCCN1CCN(CC1)CCS([O-])(=O)=O
solubility	water: 0.1 M, clear, colorless
storage condition	dry at room temperature
suitability	suitable for component for culture media
	suitable for molecular biology
technique(s)	activity assay: suitable
	cell culture \| mammalian: suitable
useful pH range	6.8-8.2

Application:	HEPES sodium salt has been used:• to supplement the HSOF medium • as a buffer component of HCZB medium in egg and embryo culture • as a buffer component in cell culture
Features and Benefits:	• High-purity product for biochemical and biological research • Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications • Highly soluble in water with a useful pH range of 6.8 - 8.2 and pKa of 7.5 at 25 °C • Suitable for Cell culture • Tested for Endotoxins and Total Aerobic Microbial Count • Free from DNase, NICKase, RNase, Endonuclease, Exonuclease and Protease
General description:	HEPES, or 4-(2-hydroxyethyl)piperazine-1-ethane-sulfonic acid, is a zwitterionic N-substituted aminosulfonic acid buffer with a remarkable pH buffering capacity between 6.8 and 8.2 (pKa of 7.48 at 25°C). Widely recognized for its versatility, HEPES is extensively employed in cell biology, biochemical, and biological research. Notably, its neutrality towards metal ions makes it a preferred choice for solutions with metal ions, acting as a "non-coordinating buffer." In cell culture, HEPES excels in maintaining stable physiological pH even amidst fluctuating carbon dioxide concentrations, outperforming bicarbonate buffers (NaHCO₃). Moreover, HEPES showcases valuable properties in various biological and biochemical processes, such as isoelectric focusing for protein separation and minimal interference in DNA-restriction enzyme reactions, making it indispensable across diverse research applications, including immunoprecipitation, cell lysis, and live cell imaging. Its versatility, exceptional buffering capacity, and minimal interaction with molecules establish HEPES as a key tool in various research domains.
Other Notes:	For additional information on our range of Biochemicals , please complete this form .
Packaging:	1 kg in poly bottle
Packaging:	25, 100, 500 g in poly bottle
Physical form:	Lyophilized, spray dried, or crystallized material.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Purity	≥99.0%
Density	1.19 g/cm³ at 20—25 °C; 1.21 g/cm³ at 20—25 °C
UNSPSC	12161700

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