Hyaluronidase from bovine testes
SIGMA/H4272 - Type IV-S, powder, suitable for mouse embryo cell culture, 750-3000 units/mg solid
Synonym: Hyaluronate 4-glycanohydrolase; Hyaluronoglucosaminidase
CAS Number: 37326-33-3
EC Number: 253-464-3
MDL Number: MFCD00131351
Product Type: Chemical
Hyaluronidase specificity: Hyaluronic acid is a polymer of alternating residues of β-D-(1-3) glucuronic acid and β-D-(1-4)-N-acetylglucosamine.
| composition | Protein, 80-105% biuret |
| form | powder |
| mol wt | ~55 kDa (four subunits of 14 kDa each) |
| Quality Level | 200  |
| specific activity | 750-3000 units/mg solid |
| sterility | aseptically processed |
| storage temp. | −20°C |
| technique(s) | cell culture | embryo: suitable |
| type | Type IV-S |
| Application: | Hyaluronidase is an enzyme derived from bovine testes. It is used to remove the cumulus mass in the isolation of mouse embryos or from newly ovulated eggs, when preparing the eggs for in vitro culture. Gonadotropins are used to superovulate 20 female mice. The mice are then mated and preimplantation stage embryos are retrieved and counted. The embryos are treated with hyaluronidase until the cumulus mass is dissociated from the embryos. The embryos are cultured, viewed daily and progress of the embryos is recorded. |
| Biochem/physiol Actions: | Hyaluronidase degrades hyaluronan and has been found to be inappropriately regulated during cancer progression. |
| Biochem/physiol Actions: | These enzymes randomly cleave β-N-acetylhexosamine-[1→4 |
| Other Notes: | One unit will degrade 0.75 μg of the polysaccharide hyaluronic acid per minute at pH 5.35 at 37 ºC (as measured by turbidimetric absorbance (λ: 600nm) when complexed with BSA after 45 minutes). |
| Preparation Note: | Reconstitute 1 vial of hyaluronidase with 3 ml of M2 Medium (Product Nos. M 7167 or M 5910) or deionized water to obtain a 10 mg/ml stock solution. Dilute stock solution with M2 Medium to obtain a final working concentration of approximately 300 μg/ml. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: 750-3000 units/mg solid |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.2.1.35 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |