EX-CELL® Hybridoma Medium
SIGMA/H4281 - without antibiotics, antimycotics, L-glutamine, and phenol red, with sodium bicarbonate, HEPES, essential and non-essential amino acids, liquid, sterile-filtered, suitable for hybridoma
Synonym: Hybridoma Medium
MDL Number: MFCD03098239
Product Type: Chemical
| components | HEPES: yes NaHCO3: yes L-glutamine: no phenol red: no glucose: 2.9 g/L |
| description | endotoxin tested |
| for research or for further manufacturing use | |
| form | liquid |
| Quality Level | 500  |
| shipped in | ambient |
| sterility | sterile-filtered |
| storage temp. | 2-8°C |
| technique(s) | cell culture | hybridoma: suitable |
| Application: | Serum-free, low-protein medium formulated to support the growth of hybridomas used in the manufacture of monoclonal antibodies. |
| Disclaimer: | Do not freeze. Protect from light. Several components of the medium are light labile and should not be exposed to light for lengthy periods of time. When stored at 2-8°C in the dark, medium that has not been supplemented is stable until the expiration date on the label. |
| Features and Benefits: | Designed to support the rapid growth of cells to high densities and the maintenance of viable cells for extended culture periods. This formulation reduces raw material cost and the low protein level reduces cost associated with downstream processing. Purified components in the product reduce the variability sometimes encountered with biological materials leading to improved consistency of the medium performance. Cells can be successfully transferred from serum-supplemented cultures to this medium with little or no adaptation. |
| Legal Information: | EX-CELL is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | This proprietary formulation includes inorganic salts, essential and non-essential amino acids, vitamins, sodium bicarbonate, HEPES, trace elements, fatty acids, and other organics. It contains low concentrations of recombinant human insulin, bovine serum albumin and human transferrin (source tested negative for HIV antibody and HbsAG). The medium does not contain |
| Preparation Note: | Prior to use, medium should be warmed to 37°C and supplemented with 50 ml/L of 200 mM |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352207 |