Collagen from human placenta
SIGMA/H4417 - Bornstein and Traub Type IV, solution, suitable for cell culture, High Performance
CAS Number: 9007-34-5
EC Number: 232-697-4
MDL Number: MFCD00130825
Product Type: Chemical
Collagen Type IV is a triple helix composed predominantly of two α1(IV) and one α2(IV), heterotrimers may be composed of six different α(IV) chains.
Bioassay Bioassay to assessing cell attachment and the bioactivity of highly purified Collagen IV CHO cells are seeded onto surfaces coated with Type IV Collagen in serum free conditions. All surfaces are blocked with a solution containing 1% BSA. Cells are then allowed to attach overnight at 37°C. The results indicate significant cell attachment bioactivity of Type IV Collagen, while the control surfaces (without collagen) show minimal or poor cell adherence. The results are presented in either Surface coverage (ug- P/cm²) or Number of attached cells per cm².
| biological source | human placenta |
| form | solution |
| impurities | Endotoxin, tested |
| HIV, hepatitis B and hepatitis C, none detected | |
| Quality Level | 200  |
| storage temp. | −20°C |
| surface coverage | <5 μg/cm2 |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P02462  |
| P08572 |
|
| P29400 |
|
| P53420 |
|
| Q01955 |
|
| Q14031 |
| Application: | Type IV collagen has been found to play a key role in angiogenesis, neurological diseases and metastasis. Collagen from human placenta has been used to assess the bioelectric effects of quinine on airway epithelial cells, to study the selective toxicity of engineered lentvirus lytic peptides and in a particle aggregation assay for the rapid detection of fibronectin, fibrinogen and collagen receptors on Staphylococcus aureus. It has also been used in a magnesium-dependent, collagen-binding assay during characterization of human lung tumor-associated antigens. |
| Biochem/physiol Actions: | During development, collagen IV is ubiquitously distributed in BMs. During the maturation process, this network gets partially replaced in a remarkably tissue specific manner, defining BM structure and function. Collagen IV has been shown to bind to platelets, hepatocytes, keratinocytes, endothelial, mesangial, pancreatic cells and some tumor cells. Tissue injury in the autoimmune disease Goodpasture syndrome is due to pathogenic autoantibodies targeting the Collagen IV α3 chain . Mutations in COL4A5 are associated with Alport syndrome. |
| Disclaimer: | Store this product at -20°C. It will retain activity for 2 years under these conditions. |
| Other Notes: | All collagen molecules are composed of three polypeptide chains arranged in a triple helical conformation, with a primary structure that is mostly a repeating motif with glycine in every third position and proline or 4-hydroxyproline frequently preceding the glycine residue. Type IV collagen occurs only in the basement membranes and contains up to six genetically distinct a-chains. |
| Other Notes: | Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Be wary of confusing Sigma-type designations with recognized collagen classification types. |
| Preparation Note: | This product is supplied as a solution in 0.5 M acetic acid. It can be used to coat tissue-culture surfaces to promote cell attachment. The amount used in these applications is dependent on the cell type, plastic ware and incubation time. A recommended amount is 0.5-5 μg/cm2. It was prepared by pepsin extraction and chromatographic purification. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 41106305 |