Hexokinase from Saccharomyces cerevisiae
SIGMA/H4502 - Type F-300, lyophilized powder, ≥130 units/mg protein (biuret)
Synonym: Hexokinase D; Hexokinasetype IV; ATP:D-
CAS Number: 9001-51-8
EC Number: 232-611-5
MDL Number: MFCD00131321
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: H4502-10KU
| anion traces | sulfate (SO42-): free |
| biological source | Saccharomyces cerevisiae |
| color | white |
| foreign activity | ATPase, myokinase, glucose-6-phosphate dehydrogenase, 6-phosphogluconic dehydrogenase and phosphoglucose isomerase ≤0.01% |
| form | lyophilized powder |
| mol wt | 110 kDa |
| purified by | crystallization |
| Quality Level | 200  |
| specific activity | ≥130 units/mg protein (biuret) |
| storage condition | (Keep container tightly closed in a dry and well-ventilated place) |
| storage temp. | −20°C |
| type | Type F-300 |
| UniProt accession no. | P04806  |
| P04807 |
| Analysis Note: | A mixture of isoenzymes. |
| Application: | Hexokinase has been used: • for digesting glucose for measuring glucose content in the root and stem samples of Quercus velutina • to treat ADP (adenosine diphosphate) solution along with • in 4-(2-hydroxyethyl)-1-pipe |
| Biochem/physiol Actions: | Catalyzes the phosphorylation of The rate of phosphorylation varies with different hexoses (pH 7.5, 30 °C). Yeast hexokinase exists as two similar isoforms, PI and PII (A and B), with isoelectric points of 5.25 and 4, respectively. Molecular Weight: ~ 54 kDa (monomer) ~110 kDa (dimer) Optimal pH: 7.5 to 9.0 Extinction Coefficient: E1% = 8.85 (PI) and 9.47 (PII) at 280 nm Activators: Hexokinase requires Mg2+ ions (KM = 2.6 mM) for activity. Hexokinase is activated by catecholamines and related compounds. Inhibitors: sorbose-1-phosphate, polyphosphates, 6-deoxy-6-fluoroglucose, 2-C-hydroxy-methylglucose |
| Biochem/physiol Actions: | Hexokinase catalyzes the rate-limiting first step of glycolysis, which involves the phosphorylation of glucose. |
| General description: | Research area: Cell Signaling The hexokinase 1 (HXK1) gene is mapped to the Saccharomyces cerevisiae chromosome VI. HXK1 is ubiquitously expressed in mammalian tissues and is abundant in the brain, erythrocytes, lymphocytes, and fibroblasts. |
| Other Notes: | One unit will phosphorylate 1.0 μmole of |
| Packaging: | 2500, 10000 units in poly bottle |
| Packaging: | 500, 1000 units in glass bottle |
| Physical form: | Lyophilized powder containing approx. 15% sodium citrate |
| Preparation Note: | Reconstitute with water or citrate buffer, pH 7.0. Studies have shown excellent stability during repeated freezing and thawing over a period of at least 30 days. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥130 units/mg protein (biuret) |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 2.7.1.1 ( BRENDA | IUBMB ) |
| UNSPSC | 12352204 |