Anti-Histone Deacetylase 11 (HDAC11) antibody produced in rabbit
SIGMA/H4539 - ~1 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-HD11; Anti-HDAC11
MDL Number: MFCD06202148
Product Type: Chemical
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 2 μg/mL Rabbit Anti-Histone Deacetylase 11 (HDAC11) (Cat. No. H4539). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. HEK-293T 2. K562 3. CHO 4. NIH-3T3 5. Neuro-2a
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~39 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot (chemiluminescent): 0.5-1 μg/mL using extracts of mouse brain |
| western blot (chemiluminescent): 4-6 μg/mL using nuclear extracts of HeLa cells. | |
| UniProt accession no. | Q96DB2  |
| Application: | Anti-Histone Deacetylase 11 (HDAC11) antibody produced in rabbit has been used in chromatin immunoprecipitation (ChIP) and western blotting analysis. |
| Biochem/physiol Actions: | Histone acetylation is a dynamic process whose levels are determined by the net activities of HATs and the competing enzymes histone deacetylases (HDACs). Histone Deacetylase 11 (HDAC11) modulates immune activation and tumorigenesis. It negatively controls the synthesis of interleukin-10 (IL-10) in antigen-presenting cells (APCs). It also modulates type I interferon (IFN) signalling. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Anti-Histone Deacetylase 11 (HDAC11) is developed in rabbit using a synthetic peptide corresponding to amino acid residues 2-16 of human HDAC11 with an N-terminal added cysteine, conjugated to KLH, as immunogen. Histone deacetylase 11 (HDAC11) belongs to the class IV member of the histone deacetylase (HDAC) family. It is mainly expressed in brain, heart, skeletal muscle, kidney, testis, immune cells and cancer cells. HDAC11 has a molecular weight of 39 kDa. The protein consists of one catalytic domain and is found in a complex with HDAC6. This gene is mapped to human chromosome 3p25. |
| Immunogen: | synthetic peptide corresponding to amino acids 2-16 of human HDAC11 with an N-terminal added cysteine, conjugated to KLH. The corresponding sequence differs by three amino acids in both rat and mouse HDAC11. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |