HMG-1 human
SIGMA/H4652 - lyophilized powder, ≥90% (SDS-PAGE), Histidine-tagged, recombinant, expressed in E. coli
Synonym: Amphoterin; HMGB1; High-Mobility-Group protein
MDL Number: MFCD03457085
Product Type: Chemical
SDS-PAGE HMG-1 Human (Cat. No. H4652) was assessed on SDS-PAGE.
| assay | ≥90% (SDS-PAGE) |
| form | lyophilized powder |
| InChI key | WQCYAHKAJFZVCO-UHFFFAOYSA |
| mol wt | 25 kDa |
| Quality Level | 200  |
| recombinant | expressed in E. coli |
| shipped in | dry ice |
| SMILES string | COC(=O)CCCCCCCCCCCCCCO |
| storage temp. | −20°C |
| UniProt accession no. | P09429  |
| Analysis Note: | Tested in DNA retardation assay |
| Application: | HMG-1 (high-mobility-group protein) human has been used: • to prepare a standard curve for measuring HMG1 using ELISA (enzyme-linked immunosorbent assay) test • to study the apoptotic ratios of Lewis cells in presence of exogenous HMG-1 • to study the changes in Th (T helper)-17 cell expansion in the presence of HMG-1 under in vitro conditions • for the migration assay of GM7373 cells |
| Biochem/physiol Actions: | HMG-1 (high mobility group protein) is a non-histone chromosomal protein, which promotes the sequence specific DNA attachment of proteins. HMG1 binds DNA in a non-sequence specific manner. It is involved in DNA replication, transcription and repair. In addition, it is up-regulated in cancers and is associated with cellular growth, vasculogenesis and apoptosis regulation. In the presence of infection or injury, HMG1 is secreted and mediates inflammation. It is associated with the pathogenesis of autoimmune diseases, such as rheumatoid arthritis (RA), inflammatory bowel disease (IBD) and systemic lupus erythematosus (SLE). |
| General description: | 215 amino acids; comprises two homologous HMG boxes, rich in basic amino acids, and an acidic tail at the carboxy-terminus |
| General description: | HMG-1 (high mobility group protein) is a nuclear protein of molecular weight 30kDa. It is secreted from macrophages, monocytes and necrotic cells. It is ubiquitously expressed. The protein has basic HMG (high mobility group) box domains and an acidic carboxyl-terminus. |
| Physical form: | Lyophilized powder containing HEPES buffer salts, pH 7.8, NaCl, EDTA, Triton X-100, Leupeptin and AEBSF. |
| Symbol |   GHS05,GHS09 |
| Signal word | Danger |
| Hazard statements | H318 - H410 |
| Precautionary statements | P273 - P280 - P305 + P351 + P338 - P391 - P501 |
| Hazard Codes | Xi |
| Risk Statements | 36-52/53 |
| Safety Statements | 26-61 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥90% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |