Monoclonal Anti-Heat Shock Protein 70 antibody produced in mouse
SIGMA/H5147 - clone BRM-22, ascites fluid
Synonym: HSP70 Antibody - Monoclonal Anti-Heat Shock Protein 70 antibody produced in mouse; Hsp70 Antibody
MDL Number: MFCD00164609
Product Type: Chemical
Western Blotting JOURNAL CITATION: Bacterial lipopolysaccharide augments febrile-range hyperthermia-induced heat shock protein 70 expression and extracellular release in human THP1 cells. By: Tulapurkar, M. E., Ramarathnam, A., et al. in PLoS One, 2015. PubMed ID: 25659128 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0118010), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Western Blotting JOURNAL CITATION: Bacterial lipopolysaccharide augments febrile-range hyperthermia-induced heat shock protein 70 expression and extracellular release in human THP1 cells. By: Tulapurkar, M. E., Ramarathnam, A., et al. in PLoS One, 2015. PubMed ID: 25659128 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0118010), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Western Blotting JOURNAL CITATION: Bacterial lipopolysaccharide augments febrile-range hyperthermia-induced heat shock protein 70 expression and extracellular release in human THP1 cells. By: Tulapurkar, M. E., Ramarathnam, A., et al. in PLoS One, 2015. PubMed ID: 25659128 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0118010), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded human prostate sections with 1:100 Monoclonal Anti-Heat Shock Protein 70, Clone: BRM-22 (Cat. No. H5147) using biotin/ExtrAvidin®-Peroxidase.
Western Blotting
Cell line lysates were separated on SDS-PAGE and probed with 1:5,000 Monoclonal Anti-Heat Shock Protein 70, Clone: BRM-22 (Cat. No. H5147). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate.
Lanes
1. A431; 2. A549; 3. NIH-3T3; 4. Hepa 1-6; 5. Mouse brain; 6. Rat brain; 7. MDBK; 8. MDCK; 9. COS-7
Cell line lysates were separated on SDS-PAGE and probed with 1:5,000 Monoclonal Anti-Heat Shock Protein 70, Clone: BRM-22 (Cat. No. H5147). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate.
Lanes
1. A431; 2. A549; 3. NIH-3T3; 4. Hepa 1-6; 5. Mouse brain; 6. Rat brain; 7. MDBK; 8. MDCK; 9. COS-7
Flow Cytometry 1×106 A549 cells were fixed and permeabilized with 2% Paraformaldehyde followed by 0.1% Triton X-100. Fixed cells were labeled with 1:100 Monoclonal Anti-Heat Shock Protein 70, Clone: BRM-22 (Cat. No. H5147) followed by Rabbit Anti-Mouse IgG, FITC conjugate. Green histogram: negative control without first antibody, Purple histogram: antibody specific staining.
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | BRM-22, monoclonal |
| conjugate | unconjugated |
| isotype | IgG1 |
| mol wt | antigen 70 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | bovine, chicken, guinea pig, plant, Drosophila, rat, human, nematode, rabbit, hamster |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | electron microscopy: suitable |
| immunohistochemistry (frozen sections): suitable | |
| indirect ELISA: suitable | |
| microarray: suitable | |
| western blot: 1:5,000 using bovine brain extract | |
| UniProt accession no. | P0DMV8  |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Immunoprecipitation (1 paper) Western Blotting (4 papers) |
| Application: | Monoclonal Anti-Heat Shock Protein 70 antibody produced in mouse has been used in: • enzyme-linked immunosorbent assay (ELISA) • immunoblot • dot blot • immunocytochemistry |
| Application: | The antibody titer of at least 1:5,000 was determined by immunoblotting using bovine brain extract. |
| Biochem/physiol Actions: | By immunoblotting, the antibody localizes both the constitutive (HSP73) and inducible (HSP72) forms of HSP70. The antibody recognizes HSP70 from brain and other tissue. Immunofluorescent staining demonstrates a rapid and reversible accumulation of the HSP70 protein within the nucleus of heat-shocked (42 °C, 1 hour) human fibroblasts. |
| Biochem/physiol Actions: | Members of the HSP70 family play a major role in the folding, unfolding and translocation of polypeptides as well as in the assembly and disassembly of oligomeric protein complexes. In addition, several possible roles have been attributed to the HSP70 family of proteins, in the immune response. It has been shown that alcoholic liver disease is associated with intracytoplasmic accumulation of HSP70. HSP72 was found to increase dramatically in the brains of Alzheimer′s disease patient, and was localized exclusively in neuritic plaques and neurofibrillary tangles. HSP70 concentrates in nuclei during heat shock and returns to the cytoplasm when the shock is removed. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | A variety of environmental disruptions, such as a sudden increase in temperature, induce cells to rapidly synthesize a group of polypeptides known as heat shock (stress) proteins. Eukaryotic cells contain a multigene family that encodes several closely related 70 kD stress proteins (the HSP70 family) that differ in their intracellular location and regulation.These include four proteins: the constitutive (or cognate) HSP73, the stress-inducible HSP72 and the glucose regulated proteins grp78 (or BiP) and grp75. |
| General description: | Monoclonal Anti-Heat Shock Protein 70 (mouse IgG1 isotype) is derived from the BRM-22 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with purified bovine brain HSP70. Heat shock protein family A (Hsp70) member 1A (HSPA1A), is encoded by the gene mapped to human chromosome 6p21.33. It is widely expressed in all tissues. The encoded protein is characterized with an N-terminal ATPase domain of 45 kDa and a C-terminal substrate binding domain of 25 kDa. |
| Immunogen: | HSP70 isolated from bovine brain |
| Other Notes: | This product can be found as purified product that was produced using cell culture hybridoma product. SAB4200714 Anti-Heat Shock Protein 70 (HSP70) antibody, Mouse monoclonal clone BRM-22, purified from hybridoma cell culture |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |