Erythropoietin human
SIGMA/H5166 - EPO, recombinant, expressed in HEK 293 cells, suitable for cell culture
Synonym: EPO
CAS Number: 11096-26-7
MDL Number: MFCD00131025
Product Type: Chemical
Bioassay EC50 is defined as the effective concentration of Erythropoietin Human that elicits a 50% attachment factor in cell based bioassay.
| assay | ≥95% (SDS-PAGE) |
| biological source | human |
| form | lyophilized powder |
| impurities | ≤1 EU/μg |
| mol wt | dimer 36 kDa (glycosylated) |
| packaging | pkg of 10 μg |
| potency | ≤5.0 ng/mL ED50 |
| quality | endotoxin tested |
| Quality Level | 100  |
| recombinant | expressed in HEK 293 cells |
| storage condition | avoid repeated freeze/thaw cycles |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P01588  |
| Analysis Note: | The specific activity was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line). |
| Biochem/physiol Actions: | Erythropoietin (EPO), produced primarily by the kidney, is the primary regulatory factor of erythropoiesis. It promotes the proliferation, differentiation, and survival of the erythroid progenitors. Erythropoietin stimulates erythropoiesis by inducing growth and differentiation of burst forming units and colony forming units into mature red blood cells. EPO produced by kidney cells is increased in response to hypoxia or anemia. The biological effects of erythropoietin are mediated by the erythropoietin receptor, which binds EPO with high affinity and is a potent EPO antagonist. |
| Biochem/physiol Actions: | Erythropoietin is a glycoprotein that is the principal regulator of red blood cell growth and differentiation. |
| General description: | EPO has been cloned from various species including human, murine, canine, and others. The mature proteins from the various species are highly conserved and exhibit greater than 80% amino acid sequence identity. EPO contains three N-linked glycosylation sites. The glycosylation of erythropoietin is required for the biological activities of erythropoietin in vivo. |
| Preparation Note: | Human EPO is expressed as a glycosylated 36 kDa monomer in human HEK 293 cells. Production in human HEK 293 cells offers authentic glycosylation. Glycosylation contributes to stability in cell growth media and other applications. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥95% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |