Histone from calf thymus
SIGMA/H5505 - Type III-S
CAS Number: 9064-47-5
MDL Number: MFCD00131329
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: H5505-100MG
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: H5505-250MG
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: H5505-25MG
| biological source | bovine (calf) thymus |
| concentration | ≥60% protein (biuret) |
| form | powder |
| Quality Level | 200  |
| solubility | H2O: soluble 10 mg/mL, clear to hazy, colorless to light yellow |
| storage temp. | 2-8°C |
| technique(s) | activity assay: suitable |
| type | Type III-S |
| Application: | Histone is suitable for use: • as a positive control in the in vitro kinase assay to examine the ability of Cdc2-cyclin B1 to phosphorylate GST-CD-4 protein • in Cdk5 activity assay • in histone H1 kinase assay • as a Poly(ADP-ribose) (PAR) acceptor in the assessment of in vitro PAR polymerase activity • as an acceptor of 32P from [γ-32P]ATP in order to measure protein kinase activity of lysate from small and large luteal cells |
| Biochem/physiol Actions: | Histones are a group of proteins characterized by high levels of lysine and arginine that form reversible complexes with DNA. Based on the fractions, the molar mass of histones varies between 11-21kDa. Five different fractions have been isolated and characterized. They include H1 (lysine rich), H2a and H2b that are slightly lysine rich, H3 and H4 that are arginine rich. H1 acts as a link between "beads" (nu bodies) on the chromatin chain. |
| Features and Benefits: | Do not confuse Sigma "Type" designations with the "Fraction" designations used by Luck and co-workers. |
| Other Notes: | Characterized as mainly subgroup f1 by SDS-PAGE |
| Packaging: | 25, 100, 250 mg in poly bottle |
| Preparation Note: | Lysine-rich fraction as isolated and described by de Nooij, E. and Westenbrink, H.G., Biochim. Biophys. Acta, 62, 608 (1962). |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 41106305 |