Anti-Histone Deacetylase 7 (HDAC7) antibody, Mouse monoclonal
SIGMA/H6663 - clone HDAC7-97, purified from hybridoma cell culture
Synonym: Anti-HDAC7
MDL Number: MFCD06798700
Product Type: Chemical
Immunoblotting Whole extract of HEK-293T cells overexpressing HDAC7 was separated on SDS-PAGE, blotted with 0.25 μg/mL Monoclonal Anti-HDAC7 Clone: HDAC7-97 (Cat. No. H6663) and developed using Goat Anti-Mouse IgG-ALP (Cat. No. A2179) and a NBT/BCIP (Cat. No. B5655) substrate.
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | HDAC7-97, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen ~105 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: suitable | |
| microarray: suitable | |
| western blot: 0.25-0.5 μg/mL using recombinant protein from embryonal kidney 293T cells over-expressing human HDAC7 | |
| UniProt accession no. | Q8WUI4  |
| Application: | Anti-Histone Deacetylase 7 (HDAC7) antibody, Mouse monoclonal has been used in: • chromatin immunoprecipitation • immunoblotting • immunocytochemistry • immunoprecipitation • ELISA |
| Application: | Monoclonal Anti-Histone Deacetylase 7 (HDAC7) antibody produced in mouse is suitable for: • immunocytochemistry • immunoprecipitation • indirect ELISA • microarray • western blot at a concentration of 0.25-0.5 |
| Biochem/physiol Actions: | HDAC7 belongs to class II of HDAC family and combines with MEF2 group of transcription factors, which is facilitated by theN-terminal domain of HDAC7. This binding blocks the transcriptional activity and prevents myogenesis. They are involved in development and differentiation processes in various tissues such as skeletal, cardiac, and smooth muscle, the bone, the immune system, the vascular system, and the brain. These signal-dependent co-repressors phosporylate at the regulatory N-terminal domain resulting in nuclear export. |
| Biochem/physiol Actions: | Shuttling of HDAC7 between the cell nucleus and the cytoplasm is controlled by a mechanism involving calmodulin dependent kinase I (CaMKI) and 14-3-3 proteins. The HDAC7 enzymatic activity depends on its interaction with the class I HDAC3, and the corepressors SMRT and N-CoR. HDAC7 also interacts with the transcriptional repressor BCL. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Histone deacetylases (HDACs) are competing enzymes, belonging to histone deacetylase family. There are two classes of HDACs with six to seven different types of HDACs proteins. HDAC1,HDAC2 and HDAC3 belong to Class I HDACs and HDAC4, HDAC6, and HDAC7 belong to Class II HDACs. Class I HDACs consists of a single deacetylase domain at the N-termini and diversified C-terminal regions, while Class II contains a deacetylase domain at C-terminal position. HDAC7 is predominantly found in the cell nucleus and slightly in cytoplasm. HDAC7 shuttles between these two regions and this is controlled by 14-3-3 protein and calmodulin-dependent kinase I (CaMKI) |
| General description: | Monoclonal Anti-Histone Deacetylase 7 (HDAC7) (mouse IgG1 isotype) is derived from the hybridoma HDAC7-97 produced by the fusion of mouse myeloma cells (NS1cells) and splenocytes from BALB/c mice. Histone deacetylases (HDACs) are part of transcriptional corepressor complexes. HDAC7 is a member of Mammalian HDAC family. It is localized mainly to the cell nucleus, it is also found in the cytoplasm. |
| Physical form: | Solution in 0.01 M phosphte buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |