HEPES sodium salt

SIGMA/H7006 - ≥99.0% (titration)

Synonym: Sodium 2-(4-(2-hydroxyethyl)piperazin-1-yl)ethanesulfonate; 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt; N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) sodium salt

CAS Number: 75277-39-3
Empirical Formula (Hill Notation): C₈H₁₇N₂NaO₄S
Molecular Weight: 260.29
EC Number: 278-169-7
MDL Number: MFCD00036463
Linear Formula: C₈H₁₇N₂NaO₄S
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-H7006-25G	25 g	$70.10 1/EA	Add To Favorites
45-H7006-100G	100 g	$166.00 1/EA	Add To Favorites
45-H7006-500G	500 g	$543.00 1/EA	Add To Favorites
45-H7006-1KG	1 kg	$992.00 1/EA	Add To Favorites
45-H7006-2.5KG	2.5 kg	$2340.00 1/EA	Add To Favorites
45-H7006-25KG	25 kg	$9260.00 1/EA	Add To Favorites
45-H7006-25KG-DP	25 kg	$9790.00 1/EA	Add To Favorites

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This image depicts SKU: H7006-100G

Properties
Descriptions
Safety Info.
Basic Data

application(s)	diagnostic assay manufacturing general analytical ( ) life science and biopharma metabolomics
assay	≥99.0% (titration)
color	white
form	powder
InChI	1S/C8H18N2O4S.Na/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14;/h11H,1-8H2,(H,12,13,14);/q;+1/p-1
InChI key	RDZTWEVXRGYCFV-UHFFFAOYSA-M
pH	10-12 (1 M in water)
pKa (25 °C)	7.5
Quality Level	400
SMILES string	[Na+].OCCN1CCN(CC1)CCS([O-])(=O)=O
solubility	water: 0.1 M, clear, colorless
storage condition	dry at room temperature
storage temp.	room temp
suitability	suitable for mass spectrometry
technique(s)	HPLC: suitable
useful pH range	6.8-8.2

Application:	HEPES buffer finds application in: • Most studies with metal ions • Substitute for Tris and phosphate in studies with metal ions • Grinding buffer in plant studies • Running buffer in gel electrophoresis • Buffer in electroporation studies • Bradford or bicinchoninic acid (BCA) assays • Extraction buffer to prevent damage of proteins in red blood cells It has been used:• as a buffer component in the preparation of a low sucrose buffer • as a buffer component in a modified Hanks′ solution, a pseudo-physiological solutions
Features and Benefits:	• Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications • Highly soluble in water • Negligible metal ion binding • Less toxic to cells than other buffers such as Tris and phosphate • Stable in a wide pH range
General description:	HEPES salt, or 4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt, is a zwitterionic biological buffer that is widely used in biological and biochemical research. It has a buffering range of pH 6.8 to 8.2, making it ideal for a variety of research applications. HEPES is also a popular choice for buffering cell culture media, offering advantages such as lower cell toxicity in comparison to alternatives like Tris and phosphate. Widely utilized in cell biology and biochemical research, HEPES proves its versatility in protein purification and extraction protocols, owing to its stability across a broad pH range and minimal metal ion binding. Additionally, HEPES proves valuable in various biological and biochemical processes, including immunoprecipitation, cell lysis, and live cell imaging, making it a versatile tool in diverse research applications.
Legal Information:	DRYPOUR is a registered trademark of Merck KGaA, Darmstadt, Germany
Legal Information:	Tyvek is a registered trademark of E. I. du Pont de Nemours and Company
Other Notes:	For additional information on our range of Biochemicals , please complete this form .
Packaging:	1 kg in poly bottle
Packaging:	2.5, 25 kg in poly drum
Packaging:	25, 100, 500 g in poly bottle
Packaging:	The DRYPOUR^®packaging system for high-quality hygroscopic salts DRYPOUR^® packaging:• The current DRYPOUR^® packaging configuration is in a 50L nestable drum • 25KG-DP are the standard prepack offering • Smaller volumes can be put in the same drum for variable configurations The benefits of DRYPOUR^®:• Improves operational efficiency by reducing material preparation time and increasing operator safety • Minimizes caking of hygroscopic salts • Offers triple protection: against moisture inside and outside, and against contamination How does the DRYPOUR^®packaging system Improve your operational efficiency?• This state-of-the-art design packaging system minimizes caking, thus significantly reducing the time needed for material preparation • PE drum with our new tamper-evident seal, a polyethylene liner with integrated desiccant bags and a breathable interior Tyvek^® liner • Desiccant bags are firmly integrated into the polyethylene liner • Triple protection delivers two invaluable results: Dramatically reduced caking No contamination risk from the desiccant
Physical form:	Lyophilized, spray dried, or crystallized material.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Purity	≥99.0% (titration)
Storage Temp.	room temp
UNSPSC	12161700

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