Hirudin from leeches
SIGMA/H7016 - lyophilized powder, ≥1,500 ATU/mg protein (ATU = antithrombin units)
CAS Number: 8001-27-2
EC Number: 232-279-1
MDL Number: MFCD00081692
Product Type: Chemical
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| biological source | animal (Hirudo verbana) |
| form | lyophilized powder |
| mol wt | 7 kDa |
| Quality Level | 100  |
| 200 |
|
| solubility | pyridine: soluble |
| water: soluble | |
| specific activity | ≥1,500 ATU/mg protein (ATU = antithrombin units) |
| storage temp. | 2-8°C |
| Analysis Note: | Protein determined by Lowry. |
| Biochem/physiol Actions: | The anticoagulant, hirudin, is the most potent natural inhibitor of both soluble and clot-bound thrombin. It binds thrombin with high affinity, covering more than 20% of its surface area and occluding both the active site and exosite I (fibrinogen and PAR recognition site). Hirudin blocks thrombus growth and platelet activation. It is not metabolized in the bloodstream of humans and is eliminated unchanged via kidney filtration. |
| Biochem/physiol Actions: | The anticoagulant, hirudin, is the most potent natural inhibitor of both soluble and clot-bound thrombin. It forms a high-affinity 1:1 complex with thrombin, occluding both the proteolytic site and exosite I (fibrinogen and PAR recognition site). Hirudin blocks thrombus growth and platelet activation, and has been suggested as the clinically preferred anticoagulant (over heparin and citrate) for its specific mode of action and absence of side effects. It is not metabolized in the bloodstream of humans and is eliminated unchanged via kidney filtration. |
| General description: | Hirudin is a 7 kDa acidic protein containing 65 amino acid residues that has an isoelectric point of 3.5-4.0. It is not glycosylated and lacks tryptophan, arginine and methionine residues. It is chemically stable between pH 2-12 and at temperatures up to 80°C. |
| Other Notes: | One unit (ATU) will neutralize one NIH unit of thrombin at 37 °C, based on direct comparison to an NIH thrombin reference standard. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥1,500 ATU/mg protein (ATU = antithrombin units) |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352202 |