Hybridization Solution
SIGMA/H7140 - for Northern and Southern blotting, liquid (2x concentrate)
Product Type: Chemical
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| foreign activity | DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected |
| form | liquid (2x concentrate) |
| Quality Level | 100  |
| storage temp. | −20°C |
| Application: | Hybridization Solution has been used to hybridise the nitrocellulose membrane. It has also been used in DNA hybridization. |
| Application: | Suitable for use as a hybridization buffer in Northern and Southern blot protocols. |
| General description: | Sigma’s Hybridization Solution is a 2x ready-to-use hybridization buffer for Northern and Southern blot protocols. |
| Preparation Note: | Sigma’s Hybridization Solution contains 200 μg/mL DNA in 10x SSC and 2X Denhardt’s solutions: 200 μg/mL DNA 2mg Bovine Serum Albumin (BSA) 2mg Ficoll 2mg Polyvinylpyrrolidone (PVP) 0.15 M sodium citrate 1.5 M NaCl |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12161700 |