Interleukin-4 human
SIGMA/H7291 - IL-4, potency:, recombinant, expressed in HEK 293 cells, HumanKine®, suitable for cell culture
MDL Number: MFCD00151705
Product Type: Chemical
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| assay | >95% (SDS-PAGE) |
| biological source | human |
| form | lyophilized powder |
| impurities | <1 EU/μg |
| manufacturer/tradename | HumanZyme |
| mol wt | dimer 14-19 kDa (glycosylated) |
| packaging | pkg of 1 mg |
| pkg of 10 μg | |
| pkg of 100 μg | |
| potency | ≤0.6 ng/mL EC50 |
| quality | endotoxin tested |
| Quality Level | 100  |
| recombinant | expressed in HEK 293 cells |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P05112  |
| Analysis Note: | The activity is determined by the dose-dependent simulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line). |
| Biochem/physiol Actions: | Interleukin-4 (IL-4) interacts with interleukin 13 and is an interleukin-4 receptor ligand. It regulates IgE and IgG1 production by B cells. IL-4 favors glial and neuronal differentiation and regulates differentiation of naive cluster of differentiation CD4+ T cells to the T helper type 2 (TH2) cells. Polymorphism in the IL-4 gene is implicated in immune and inflammatory disorders. In cerebral infarction, IL-4 levels are elevated. |
| General description: | Interleukin-4 (IL-4) is a pleiotropic cytokine and a complex glycoprotein mapped to human chromosome 5q31.1. It is produced by mast cells, basophils and a subset of activated T cells. |
| Legal Information: | HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc |
| Packaging: | 10 μg in poly bottle |
| Physical form: | Lyophilized from a 0.2 μm filtered solution of 1x PBS. |
| Preparation Note: | HumanKine Interleukin-4 (IL-4), expressed in human HEK 293 cells, is a glycosylated monomer with an apparent molecular mass of 14-19 kDa due to glycosylation, which is absent when this cytokine is expressed in E. coli. Production in human HEK 293 cells offers authentic glycosylation. Glycosylation contributes to stability in cell growth media and other applications. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | >95% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |