Anti-HA−FITC antibody, Mouse monoclonal
SIGMA/H7411 - clone HA-7, purified from hybridoma cell culture
Synonym: Monoclonal Anti-HA; Anti-HA; Anti-Influenza Hemagglutinin
MDL Number: MFCD00803873
Product Type: Chemical
Immunofluorescence Whole extract of 293T cells expressing HA fusion protein were fixed and permeabilized with 3% to 4% paraformaldehyde followed by 0.5% Triton™ X-100. Fixed cells were stained with 1 μg/mL Anti-HA-FITC antibody, Mouse monoclonal, Clone: HA-7 (Cat. No. H7411).
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | HA-7, monoclonal |
| concentration | 1 mg/mL |
| conjugate | FITC conjugate |
| form | buffered aqueous solution |
| Quality Level | 200  |
| shipped in | wet ice |
| storage condition | protect from light |
| storage temp. | −20°C |
| technique(s) | immunocytochemistry: 1-5 μg/mL using HA tagged fusion protein transfected into mammalian cells fixed with paraformaldehyde. |
| Application: | Antibody suitable for Immunofluorescence. Recommended concentration 1-5 mg/ml. For immunocytochemistry, use HA tagged fusion protein transfected into mammalian cells, then fixed with paraformaldehyde. Anti-HA-FITC antibody, Mouse monoclonal has been used: • in the labeling of HA-tagged transient receptor potential melastatin 7 (TRPM7) channel protein for immunocytochemistry studies in lymphoblast cell DT40 and human embryonic kidney cells (HEK-293) cells • in western blotting detection of spindle-associated proteins in Trypanosoma brucei • in immunostaining of vascular smooth muscle cells (SMCs) for immunocytofluorescence studies |
| Biochem/physiol Actions: | Hemagglutinin (HA) induces immune response post viral infection. Fluorescein isothiocyanate (FITC) conjugated with the influenza virus HA is majorly used in intracellular tracking of the virus and also in flow cytometric studies. |
| Biochem/physiol Actions: | The antibody recognizes native as well as denatured-reduced forms of HA-tagged proteins and is reactive with N- or C-terminal HA-tagged fusion proteins expressed in E. coli or in mammalian cells. |
| General description: | Anti HA-FITC is a mouse monoclonal antibody derived from the HA-7 hybridoma. It is isolated from ascites fluid and conjugated to fluorescein isothiocyanate, isomer I (FITC). The antibody recognizes the HA tag expressed on the N- or C-terminus on HA tagged H7411proteins. The antibody reacts specifically with HA tagged proteins by immunocytochemistry. |
| General description: | Hemagglutinin (HA) is the major envelope glycoprotein of the influenza virus, along with neuraminidase. |
| Immunogen: | synthetic peptide corresponding to a fragment of human influenza virus hemagglutinin (HA) known as HA-tag, conjugated to KLH |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide as a preservative. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |