HEPES
SIGMA/H7523 - BioXtra, pH 5.0-6.5 (1 M in H2O), ≥99.5% (titration)
Synonym: 4-
CAS Number: 7365-45-9
Empirical Formula (Hill Notation): C8H18N2O4S
Molecular Weight: 238.30
EC Number: 230-907-9
MDL Number: MFCD00006158
Linear Formula: C8H18N2O4S
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.
This image depicts SKU: H7523-50G
This image depicts SKU: H7523-50G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.
This image depicts SKU: H7523-250G
This image depicts SKU: H7523-250G
| absorption | ≤0.035 at 280 in H2O at 1 M |
| ≤0.045 at 260 in H2O at 1 M | |
| anion traces | chloride (Cl-): ≤0.05% |
| application(s) | diagnostic assay manufacturing |
| assay | ≥99.5% (titration) |
| cation traces | Al: ≤0.0005% |
| As: ≤0.0001% | |
| Ba: ≤0.0005% | |
| Bi: ≤0.0005% | |
| Ca: ≤0.001% | |
| Cd: ≤0.0005% | |
| Co: ≤0.0005% | |
| Cr: ≤0.0005% | |
| Cu: ≤0.0005% | |
| Fe: ≤0.0005% | |
| K: ≤0.005% | |
| Li: ≤0.0005% | |
| Mg: ≤0.0005% | |
| Mn: ≤0.0005% | |
| Mo: ≤0.0005% | |
| Na: ≤0.005% | |
| Ni: ≤0.0005% | |
| Pb: ≤0.0005% | |
| Sr: ≤0.0005% | |
| Zn: ≤0.0005% | |
| form | powder |
| ign. residue (900°C) | ≤0.1% (as SO4) |
| impurities | Insoluble matter, passes filter test |
| InChI | 1S/C8H18N2O4S/c11-7-5-9-1 |
| InChI key | JKMHFZQWWAIEOD-UHFFFAOYSA |
| loss | ≤1.0% loss on drying, 110°C |
| pH | 5.0-6.5 (1 M in H2O) |
| pKa (25 °C) | 7.5 |
| product line | BioXtra |
| Quality Level | 400  |
| SMILES string | OCCN1CCN(CC1)CCS(O)(=O)=O |
| solubility | H2O: 1 M, clear, colorless |
| useful pH range | 6.8-8.2 |
| Application: | HEPES has been used: • As a component of homogenisation buffer used in protein sample preparation • To neutralise PRP (platelet-rich plasma) solution as part of PRP preparation • As a component in 2X PBS buffer, which is used in phosphate calcium transfection in cell line preparation • As a supplement in RPMI 1640 medium used to culture human umbilical vein endothelial cells (HUVEC) and in M199 complete medium for ex-vivo treatment of testicular tissue. |
| General description: | HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg. |
| Other Notes: | Easily compare specifications for HEPES products with the HEPES specification table . |
| Packaging: | 50, 250 g in poly bottle |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥99.5% (titration) |
| UNSPSC | 12161700 |