Anti-Huntingtin (N-terminal) antibody produced in rabbit
SIGMA/H7540 - affinity isolated antibody, buffered aqueous solution
Synonym: Anti-HD; Anti-HTT; Anti-Huntington Disease; Anti-IT15
Product Type: Product-on-demand
Immunocytochemistry-immunoflourescence JOURNAL CITATION: A genome-scale RNA-interference screen identifies RRAS signaling as a pathologic feature of Huntington′s disease. By: Miller, J. P., Yates, B. E., et al. in PLoS Genet, 2012. PubMed ID: 23209424 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pgen.1003042), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunohistochemistry-immunofluorescence JOURNAL CITATION: A genome-scale RNA-interference screen identifies RRAS signaling as a pathologic feature of Huntington′s disease. By: Miller, J. P., Yates, B. E., et al. in PLoS Genet, 2012. PubMed ID: 23209424 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pgen.1003042), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Enhanced Validation-By Recombinant Expression Antibody recognition: Anti-Huntingtin (N-terminal) antibody produced in Rabbit (Cat. No. H7540) is confirmed by western blot in the cell sample that has been altered to overexpress the target protein-HEK-293T over expressing Huntingtin (Lane 1) while no staining is detected in the wild type sample-HEK-293T (Lane 2). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Antibody dilution: 1:1,000.
Immunoblotting Cell lysates of HEK-293T overexpressing human huntingtin N-terminal fragment (Lane 1,3) and HEK-293T non-transfected (Lane 2), were separated on SDS-PAGE, blotted with Anti-Huntingtin (N-terminal) (Cat. No. H7540) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and chemiluminescent substrate. Lanes 1. Antibody: 0.5 μg/mL 2. Antibody: 0.5 μg/mL 3. Antibody: 0.5 μg/mL + huntingtin immunizing peptide (human, 3-16)
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.5 mg/mL |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| mol wt | antigen ~110 kDa |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | mouse, human, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 0.5-1.0 μg/mL using a HEK-293T cell lysate expressing a N-terminal fragment of human huntingtin |
| UniProt accession no. | P42858  |
| Application: | Anti-Huntingtin (N-terminal) antibody has been used: • in magnetic bead-assisted immunopurification • in western blotting • in immunofluorescent staining • in immunoblotting |
| Biochem/physiol Actions: | Huntingtin (Htt) is essential for embryonic development and neurogenesis. Htt associates with various cell organelles like the nucleus, endoplasmic reticulum and Golgi complex. It is also found in neurites and synapses where it associates with vesicular structures and microtubules, suggesting that it may play a role in intracellular vesicular trafficking, calcium homeostasis, neuronal survival, morphogenesis and transcriptional regulation. Aberrant expansion of glutamine repeats (polyQ) in the N-terminal region of huntingtin (htt) causes Huntington′s disease (HD) which is an autosomal dominant neurodegenerative disorder. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Anti-Huntingtin (N-terminal) is produced in rabbit using as immunogen a synthetic peptide corresponding to human huntingtin, conjugated to KLH. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |