Heregulin-β1 (EGF Domain) human
SIGMA/H7660 - ≥98% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder
Synonym: HRG-β1
MDL Number: MFCD03457066
Product Type: Chemical
Bioassay Basic growth factor is a protein that is mitogenic for a variety of cell types. HRG (EGF Domain) growth factor potency is quantified by its ability to stimulate a growth of Balb/3T3 cells in serum free conditions. The relative cell growth is detected using a fluorimetric assay based on the measurement of the conversion of Resazurin to Resorufin by metabolically active cells. The amount of HRG (EGF Domain) that will give 50% of the maximal proliferation (EC50) is calculated from the different amounts assayed.
| assay | ≥98% (SDS-PAGE) |
| biological source | human |
| color | white |
| form | lyophilized powder |
| impurities | endotoxin, tested |
| mol wt | predicted mol wt 8 kDa |
| packaging | pkg of 10 and 50 μg |
| potency | <0.50 ng/mL |
| Quality Level | 200  |
| recombinant | expressed in E. coli |
| storage condition | avoid repeated freeze/thaw cycles |
| storage temp. | −20°C |
| UniProt accession no. | Q02297  |
| Analysis Note: | The biological activity is measured by its ability to stimulate the proliferation of human MCF-7 cells grown under serum-free conditions. |
| Biochem/physiol Actions: | Growth factor ligand for ErbB3 and ErbB4 receptor tyrosine kinases. |
| Biochem/physiol Actions: | Growth factor ligand for ErbB3 and ErbB4 receptor tyrosine kinases. The binding of HRG results in receptor dimerization and receptor trans-autophosphorylation |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥98% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |