Anti-Histone H1.4 antibody produced in rabbit
SIGMA/H7665 - ~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-H1 Histone family, member 4; Anti-H1.4; Anti-H1F4; Anti-HIST1H1E; Anti-Histone H1b; Anti-Histone I family, 4; Anti-Histone cluster 1 H1e
Product Type: Chemical
Immunofluorescence HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton™ X-100. Fixed cells were stained with 5 μg/mL Rabbit Anti-Histone H1.4 (Cat. No. H7665). The antibody was developed using Goat Anti-Rabbit IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
Immunoblotting HL60 cells extract (acid-extracted fraction) (Lanes 1,2), was separated on SDS-PAGE, blotted with Anti-Histone H1.4 (Cat. No. H7665) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and chemiluminescent substrate. Lanes 1. Antibody: 1.5 μg/mL 2. Antibody: 1.5 μg/mL + Histone H1.4 immunizing peptide (human, 22-33)
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~35 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 1-2 μg/mL using acid-extracted fraction of HL60 cells |
| UniProt accession no. | P10412  |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Western Blotting (1 paper) |
| Biochem/physiol Actions: | Linker histone H1 binds to DNA between nucleosomal core particles and is involved in establishing and maintaining higher order chromatin structures. It is covalently modified. Histone H1 phosphorylation occurs at multiple sites including at Ser27 residue. Histone H1.4 is di-methylated or acetylated at Lys26. enhancer of Zeste 2 (Ezh2). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | In mammalian cells, four histone H1 variants (H1.2 to H1.5) are found in all somatic cells, and a fifth (H1.1) is present in thymus, testis and spleen, lymphocytic and neuronal cells. K26/H1.4 is present within the flexible N-terminal domain of H1.4 just before the globular domain. Histone modifications are thought to play an important role in cancer and disease. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |