Interleukin-12 human
SIGMA/H7666 - HumanKine®, recombinant, expressed in HEK 293 cells, suitable for cell culture, endotoxin, tested
Synonym: Interleukin-12 human; IL-12
MDL Number: MFCD00212975
Product Type: Chemical
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| assay | ≥95% (SDS-PAGE) |
| biological source | human |
| form | lyophilized |
| impurities | endotoxin, tested |
| mol wt | dimer 57 kDa (glycosylated) |
| packaging | pkg of 10 μg |
| potency | ≤2 ng/mL EC50 |
| Quality Level | 200  |
| recombinant | expressed in HEK 293 cells |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| Analysis Note: | The specific activity was determined by the dose-dependent release of IFN-γ from the human NK92 cell line in presence of 20 ng/mL rIL-2. |
| Application: | Interleukin-12 (IL-12) human has been used to study IL-12 mediated enhancement of IL-12Rβ2 (IL-12 receptor) expression. |
| Biochem/physiol Actions: | Interleukin-12 (IL-12) was identified as a factor secreted by human Epstein-Barr (EBV)-transformed B cell lines. IL-12, or Natural Killer Cell Stimulatory Factor (NKSF), is a 75 kDa disulfide-linked heterodimer of a 35 kDa subunit and 40 kDa subunit. IL-12 is produced predominantly by monocytes and NK cells and induces T cells and NK cells to produce IFN-γ. Human IL-12 is not active on mouse cells, but murine IL-12 is active on both murine and human lymphocytes. The biological activity of recombinant human IL-12 was measured by its ability to stimulate the proliferation of PHA-activated human T lymphoblasts. IL-12 has anti-angiogenic properties. |
| Legal Information: | HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc |
| Physical form: | lyophilized from a 0.2 μm filtered solution of PBS |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥95% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |