Hygromycin B from Streptomyces hygroscopicus

SIGMA/H7772 - lyophilized powder

Synonym: Hygrovetine

CAS Number: 31282-04-9
Empirical Formula (Hill Notation): C₂₀H₃₇N₃O₁₃
Molecular Weight: 527.52
EC Number: 250-545-5
MDL Number: MFCD06795479
Linear Formula: C₂₀H₃₇N₃O₁₃
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-H7772-50MG	50 mg	$44.60 1/EA	Add To Favorites
45-H7772-100MG	100 mg	$70.50 1/EA	Add To Favorites
45-H7772-250MG	250 mg	$155.00 1/EA	Add To Favorites
45-H7772-1G	1 g	$235.00 1/EA	Add To Favorites

This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: H7772-100MG

Properties
Descriptions
Safety Info.
Basic Data

antibiotic activity spectrum	viruses
biological source	Streptomyces hygroscopicus
color	white to beige
	faintly brown to brown
form	lyophilized powder
InChI	1S/C20H37N3O13/c1-23-7-2-5(21)9(26)15(10(7)27)33-19-17-16(11(28)8(4-25)32-19)35-20(36-17)18(31)13(30)12(29)14(34-20)6(22)3-24/h5-19,23-31H,2-4,21-22H2,1H3/t5-,6-,7+,8-,9+,10-,11+,12-,13+,14-,15-,16+,17+,18-,19+,20+/m1/s1
InChI key	GRRNUXAQVGOGFE-XKIAHZFYSA-N
mode of action	protein synthesis \| interferes
purified by	ion-exchange chromatography
Quality Level	200
SMILES string	CN[C@H]1C[C@@H](N)[C@H](O)[C@@H](O[C@@H]2O[C@H](CO)[C@H](O)[C@@H]3O[C@]4(O[C@H]([C@H](N)CO)[C@H](O)[C@H](O)[C@H]4O)O[C@H]23)[C@@H]1O
solubility	H₂O: 50 mg/mL
storage temp.	2-8°C

Application:	Hygromycin B is an aminoglycoside antibiotic isolated from Streptomyces hygroscopicus. It has been used to study protein synthesis at the level of the 70S ribosome translocation and mRNA template misreading, as an antiviral agent by selectively penetrating cells rendered permeable by virus infection and inhibiting translation, and as a selection agent for hygromycin resistance gene transformed cells. It is recommended for use as a selection agent at 100-800 μg/mL, specifically at 100 μg/mL for prokaryotes, 200 μg/mL for lower eukaryotes and 150-400 μg/mL for higher eukaryotes..
Biochem/physiol Actions:	Mode of Action: The product acts by inhibiting protein synthesis by inducing the misreading of the m-RNA template in the prokaryote, with the potency to inhibit translation. Antimicrobial Spectrum: Hygromycin B acts against bacteria, fungi and higher eukaryotic cells.
Disclaimer:	Hygromycin B products should be stored as supplied at 2-8°C, and the dry solid is stable for at least 5 years if stored at 2-8°C. It is stable at 37°C for 30 days.
General description:	Chemical structure: aminoglycoside
Packaging:	1 g in glass bottle
Preparation Note:	This product is purified by ion exchange chromatography. Hygromycin B is soluble in water at concentrations >50 mg/mL, and also soluble in methanol or ethanol. Solutions should be sterilized by filtration, not by autoclaving. Additionally, Hygromycin B solutions have been reported to lose activity on freezing, and since they are stable refrigerated, freezing should be avoided.

Symbol	GHS06
Signal word	Danger
Hazard statements	H300 + H310 + H330
Precautionary statements	P260 - P262 - P264 - P280 - P302 + P352 + P310 - P304 + P340 + P310
Hazard Codes	T+
Risk Statements	26/27/28-41-42/43
Safety Statements	22-26-28-36/37/39-45
RIDADR	UN3462 - class 6.1 - PG 1 - Toxins, extracted from living source
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Storage Temp.	2-8°C
UNSPSC	51102829

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