HIS-Select® Spin Columns
SIGMA/H7787
Synonym: His-Tag Spin Columns
Product Type: Chemical
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| capacity | >0.5 mg/column |
| packaging | pkg of 10 ea |
| pkg of 50 ea | |
| pore size | 100 nm pore size |
| Quality Level | 200  |
| shelf life | 2 yr at 2‑8 °C |
| storage temp. | 2-8°C |
| Application: | Pre-packed and ready-to-use HIS-Select Spin Columns allow for fast and consistent purification of small-scale crude cell extracts containing histidine-tagged proteins. The spin columns contain a matrix of silica particles and uses the HIS-Select patented non-charged, hydrophilic nickel chelating linkage chemistry. HIS-Select Spin Columns are highly selective for histidine-tagged proteins and exhibit very low non-specific binding of other proteins. These convenient Spin Columns allow for fast, one-step purification for the isolation of at least 150 μg of histidine-tagged protein in less than 15 minutes. |
| Features and Benefits: | • High Selectivity for higher purity • Convenient and Ready-to-Use • Consistent protein binding each use • Compatible with CelLytic™ cell lysis reagents |
| General description: | Kit contains 3 collection tubes for every spin column. |
| Legal Information: | CelLytic is a trademark of Sigma-Aldrich Co. LLC |
| Legal Information: | HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 41106500 |