Hanahan’s Broth (SOB Medium)
SIGMA/H8032 - powder microbial growth medium
Synonym: Super Optimal Broth
Product Type: Chemical
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| application(s) | food and beverages microbiology |
| composition | KCl, 0.186 g/L |
| MgSO4, 2.4 g/L | |
| NaCl, 0.5 g/L | |
| Tryptone, 20 g/L | |
| Yeast extract, 5 g/L | |
| description | recommended for cultivation of recombinant E. coli. |
| form | powder |
| grade | Molecular Biology |
| pH | 6.8-7.2(2.8% solution) |
| Quality Level | 100  |
| sterility | non-sterile |
| storage temp. | room temp |
| suitability | nonselective for Escherichia coli |
| nonselective for coliforms | |
| technique(s) | microbiological culture: suitable |
| Application: | Hanahan′s Broth is recommended for use in cultivation of recombinant strains of Escherichia coli. It is suitable for the preparation of competent E. coli., often with the addition of 0.4% dextrose. It has also been used for the preparation of C. necator H16 competent cells. It is suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. |
| Features and Benefits: | Hannahan’s Broth (SOB) powder provides: • Standard formulation • An inexpensive alternative to liquid |
| General description: | Hannahan’s Broth (SOB) is a microbial growth medium used for the preparation and transformation of competent cells (E. coli). Casein enzymic hydrolysate and yeast extract supply nitrogenous compounds and growth factors for recombinant Escherichia coli. Potassium chloride and sodium chloride maintain the isotonic condition. Magnesium sulfate is added to the medium as a necessary component for DNA replication. |
| Other Notes: | Casein enzymic hydrolysate and yeast extract supply the necessary nitrogenous compounds and growth factors. Potassium chloride and sodium chloride provide an isotonic environment for the cells. Magnesium sulfate aids DNA replication. |
| Preparation Note: | Stir to suspend 28 g powder in 1L water. Autoclave for 15 minutes at 121°C to sterilize. Allow to cool before making additions, such as antibiotics (if desired). |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | room temp |
| UNSPSC | 41106200 |