HIS-Select® Cobalt Affinity Gel
SIGMA/H8162 - (1:1 suspension in a 30% ethanol solution)
Synonym: cobalt affinity gel; cobalt charged agarose
Product Type: Chemical
| capacity | >15 mg/mL, agarose binding capacity (protein)(with an approx. 30 kDa protein) |
| form | (1:1 suspension in a 30% ethanol solution) |
| matrix | 6% Beaded Agarose |
| Quality Level | 200  |
| shelf life | 1 yr (when stored properly.) |
| storage temp. | 2-8°C |
| technique(s) | protein purification: suitable |
| Application: | HIS-Select Cobalt Affinity Gel allows for high purity, low non-specific binding, and high binding capacity of protein. HIS-Select Cobalt Affinity Gel also works well for purification of histidine-tagged proteins / His-tag proteins in native, denaturing, or mild reducing conditions. HIS-Select® Cobalt Affinity Gel has been used to purify His-tagged proteins. |
| General description: | The HIS-Select Cobalt Affinity Gel is a quadridentate chelate on beaded agarose that is charged with cobalt. The gel is designed to specifically bind histidine-containing proteins / His-tag proteins. It is selective for recombinant proteins with histidine tags and exhibits low non-specific binding of other proteins. HIS-Select Cobalt Affinity Gel is an immobilized metal-ion affinity chromatography (IMAC) product. The matrix for this affinity gel is 6% beaded agarose. The selectivity can be modulated with the inclusion of imidazole during chromatography. HIS-Select Cobalt Affinity Gel is durable and can capture recombinant proteins with histidine tags at a high flow rate. Recombinant proteins with His tags are bound using native, denaturing, or mild reducing conditions. The capacity of this affinity gel is typically >15 mg/mL of packed gel, determined with a His-tagged protein of molecular mass ~30 kDa. |
| Legal Information: | HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Packaging: | 5, 100 mL in poly bottle |
| Physical form: | 1:1 suspension in a 30% ethanol solution |
| Symbol |     GHS02,GHS07,GHS08,GHS09 |
| Signal word | Danger |
| Hazard statements | H226 - H302 - H317 - H334 - H341 - H350 - H360D - H410 |
| Precautionary statements | P201 - P210 - P273 - P280 - P301 + P312 + P330 - P308 + P313 |
| Hazard Codes | T,N |
| Risk Statements | 49-60-10-22-42/43-50/53-68 |
| Safety Statements | 53-23-36/37-45-61 |
| RIDADR | UN1170 - class 3 - PG 3 - Ethanol, solution |
| WGK Germany | WGK 3 |
| Flash Point(F) | 118.4 °F - closed cup - Not |
| Flash Point(C) | 48 °C - closed cup - Not ap |
| Storage Temp. | 2-8°C |
| UNSPSC | 41106500 |