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N-Hydroxysuccinimidyl-Sepharose™ 4 Fast Flow

SIGMA/H8280 - matrix fast-flow highly cross-linked 4% beaded agarose

Synonym: N-Hydroxysuccinimidyl-Agarose

MDL Number: MFCD00164950
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-H8280-5ML 5 mL
 $157.00
1/EA		 Add To Favorites
45-H8280-15ML 15 mL
 $343.00
1/EA		 Add To Favorites
Prepared by coupling Sepharose with 6-aminohexanoic acid via a spacer arm. The resulting terminal carboxyl group is activated by esterification with N-hydroxysuccinimide. Ligands containing primary amino groups couple directly to the active ester.

 

extent of labeling 16-23 μmol per mL
form isopropanol suspension
matrix fast-flow highly cross-linked 4% beaded agarose
matrix active group N-hydroxysuccinimide active ester
matrix spacer 6 atoms (when ligands are coupled by displacement of NHS)
Quality Level 200 
storage temp. 2-8°C
technique(s) protein array: suitable
Application: N-Hydroxysuccinimidyl-Sepharose™ 4 Fast Flow has been used as a part of a magnetic particles-based visual detection system to achieve separation upon binding of the amplified gene. It has also been used to immobilize protein during pull-down assay.
Features and Benefits: • Specifically recommended for coupling of small amino-containing proteins and peptides in process-scale applications
• High level of pre-activation, which gives a high degree of substitution of the selected ligand
• NHS coupling method enables chemically stable ligand attachment
General description: N-Hydroxysuccinimidyl-Sepharose™ 4 Fast Flow is a cross-linked and preactivated matrix obtained by mixing Sepharose 4 Fast Flow with 6-aminohexanoic acid through a spacer arm. Esterification with N-hydroxysuccinimide activates the terminal carboxyl group. Ligand-containing primary amino groups directly interact with this active ester to form a chemically very stable amide linkage.
Legal Information: Sepharose is a trademark of Cytiva
Storage Temp. 2-8°C
UNSPSC 23151817

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