Stem Cell Factor human
SIGMA/H8416 - SCF, recombinant, expressed in HEK 293 cells, suitable for cell culture
Synonym: SCF; c-Kit ligand
MDL Number: MFCD00165757
Product Type: Chemical
The biological activity of SCF human, recombinant (Cat. No. H8416) was assessed in a cell proliferation assay using TF1 cells.The EC50 is defined as the effective concentration that elicits a 50% increase in cell proliferation in a cell-based bioassay.
The purity of SCF human, recombinant (Cat. No. H8416) was assessed on SDS-PAGE stained with InstantBlue™ (Cat. No. ISB1L).
| assay | ≥95% (SDS-PAGE) |
| biological source | human |
| form | lyophilized powder |
| impurities | ≤1 EU/μg |
| mol wt | dimer 35-45 kDa (glycosylated) |
| packaging | pkg of 10 μg |
| pkg of 100 μg | |
| pkg of 5x10 μg | |
| potency | ≤200 ng/mL ED50 |
| quality | endotoxin tested |
| Quality Level | 200  |
| recombinant | expressed in HEK 293 cells |
| storage condition | avoid repeated freeze/thaw cycles |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P21583  |
| Analysis Note: | The specific activity was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line). |
| Biochem/physiol Actions: | Stem cell factor (SCF) of granulosa cell origin, efficiently contributes to the follicular development and oocyte maturation. SCF controls tumor development and progression. Elevated SCF gene expression is observed in hepatocellular carcinoma. |
| Biochem/physiol Actions: | Stem Cell Factor (SCF), also known as c-Kit ligand (KL), steel factor (SLF) and mast cell growth factor (MGF), is a 30 kDa glycoprotein with broad activities on various tissues, including hematopoietic cells, pigment cells, and primordial germ cells. |
| Biochem/physiol Actions: | Stem Cell Factor (SCF), also known as c-Kit ligand (KL), steel factor (SLF) and mast cell growth factor (MGF), is a 30 kDa glycoprotein with broad activities on various tissues, including hematopoietic cells, pigment cells, and primordial germ cells. SCF is secreted by endothelial cells, fibroblasts, and bone marrow stromal cells as a membrane-bound form which may be cleaved to release the soluble form. Both forms are active in promoting colony formation from murine bone marrow cells, but membrane-bound SCF is more effective in promoting hematopoieses in vivo, suggesting a role in cellular interactions between hematopoietic and stromal cells. The soluble is thought to exist in solution as a noncovalently linked dimer. SCF is structurally related to M-CSF (CSF-1) and Flt-3/Flk-2 Ligand (FL) with all three sharing a similar size, existence of transmembrane and soluble forms, four conserved cysteines, and alternative splicing exon locations, but they share little sequence homology. SCF alone is a modest colony stimulating factor. However, in the presence of other cytokines such as EPO, TPO, GM-CSF, G-CSF, M-CSF, IL-3, and IL-7, SCF is a potent costimulant that works synergistically to increase the size of myeloid, erythroid or lymphoid lineage colonies without influencing the lineage differentiation of the progenitors. |
| General description: | The KITLG (KIT ligand) gene is mapped to human chromosome 12q21.32. |
| Legal Information: | HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc |
| Packaging: | 10 μg in poly bottle |
| Preparation Note: | SCF is expressed in human HEK 293 cells as a glycosylated monomer with an apparent molecular mass of 35-45 kDa due to glycosylation. Production in human 293 cells offers authentic glycosylation. Glycosylation contributes to stability in cell growth media and other applications. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥95% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |