Transforming Growth Factor-β1 human
SIGMA/H8541 - TGF-β1, Xeno-free, recombinant, expressed in HEK 293 cells, suitable for cell culture
Synonym: TGF-β1
MDL Number: MFCD00166089
Product Type: Chemical
TGF-beta Signaling
Research areas: Apoptosis, Differentiation, Epithelial Mesenchymal transition, Cancer biology, Bone remodelling, Developmental biology, Inflammation, Metabolism
Disease areas: Gastrointestinal cancers, Pulmonary, liver and renal fibrosis, Diabetes, Tuberculosis
See also: T7039; H8541; B3555; 11412272001; H4791; H8791
Research areas: Apoptosis, Differentiation, Epithelial Mesenchymal transition, Cancer biology, Bone remodelling, Developmental biology, Inflammation, Metabolism
Disease areas: Gastrointestinal cancers, Pulmonary, liver and renal fibrosis, Diabetes, Tuberculosis
See also: T7039; H8541; B3555; 11412272001; H4791; H8791
| assay | ≥95% (SDS-PAGE) |
| biological source | human |
| form | lyophilized powder |
| impurities | ≤1 EU/μg |
| mol wt | dimer 25 kDa (non-glycosylated) |
| packaging | pkg of 5 μg |
| potency | ≤0.5 ng/mL EC50 |
| quality | endotoxin tested |
| Quality Level | 300  |
| recombinant | expressed in HEK 293 cells |
| storage condition | avoid repeated freeze/thaw cycles |
| storage temp. | -10 to -25°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P01137  |
| Analysis Note: | The activity was determined by the dose dependent inhibition of IL-4 induced proliferation of mouse HT-2 cells (BALB/c spleen activated by sheep erythrocytes in the presence of IL-2). |
| Application: | Transforming Growth Factor-β1 (TGF-β) human has been used : • to study the expression of PDGFRB (Platelet derived growth factor receptor β) in association with transforming growth factor-β signaling. • to stimulate lung fibrosis in human fetal lung fibroblast cell line in order to further measure the in-plane elasticity of live cell layers using a pressure sensor embedded microfluidic device. • to study the effect of TGF-β on HTRA1 (high-temperature requirement A1)-affected Wnt target genes. |
| Biochem/physiol Actions: | TGF-β1 is a multifunctional peptide capable of influencing cell proliferation, differentiation, and other functions in a wide range of cell types. Transformed as well as non-neoplastic tissues release transforming growth factors and essentially all cells possess a specific TGF-β1 receptor. In general, cells of mesenchymal origin appear to be stimulated by TGF-β1; whereas, hepatocytes, T and B lymphocytes, keratinocytes, and many epithelial cells are inhibited by the peptide. TGF-β1 interacts with Epidermal Growth Factor (EGF), Platelet Derived Growth Factor (PDGF), Fibroblast Growth Factor (FGF), and T Cell Growth Factor (T-CGF) either by enhancing or antagonizing their characteristic actions. TGF-β1 plays a fundamental role in tissue growth and differentiation by involvement in adipogenesis, myogenesis, chondrogenesis, osteogenesis, epithelial cell differentiation, and immune cell function. TGF-β1 is associated with the developmental and metastasis of colorectal cancer. |
| Biochem/physiol Actions: | Transforming growth factor-β1 (TGF-β1) is produced by many cell types, but is reported to be most concentrated in mammalian platelets, where it is present at approximately four times the level of TGF-β2. |
| General description: | The TGFB1 gene is mapped to human chromosome 19q13.2 and codes for a 25 kDa peptide. TGF-ß1 polypeptide is produced as a precursor with hydrophobic signal sequence, pro-region and mature peptide. Cleavage of the precursor at tetrabasic cleavage site results in monomers of carboxy-terminal 112 amino acids. The biologically active form of Tgfb1 exists as a disulphide-linked homodimer. |
| Packaging: | 5 μg in poly bottle |
| Physical form: | Lyophilized from a 0.2 μm filtered solution of 50 mM NaOAc pH 4.5 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥95% (SDS-PAGE) |
| Storage Temp. | -10 to -25°C |
| UNSPSC | 12352202 |