Hexokinase and Glucose-6-phosphate Dehydrogenase from baker’s yeast (S. cerevisiae)
SIGMA/H8629 - lyophilized powder
Synonym: HK/G6PD
MDL Number: MFCD00131322
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: H8629-5KU
| form | lyophilized powder |
| glucose-6-phosphate dehydrogenase activity | ≥75 units/mg protein (biuret) |
| hexokinase activity | ≥65 units/mg protein |
| purified by | crystallization |
| Quality Level | 200  |
| storage temp. | −20°C |
| Application: | Hexokinase and Glucose-6-phosphate Dehydrogenase from baker′s yeast (S. cerevisiae) has been used to evaluate the adenylate kinase (Adk), activities of the mutant Adk’s. It has also been used for the determination of glucose and ATP. |
| Biochem/physiol Actions: | Hexokinase initiates the glycolysis pathway by catalyzing the phosphorylation of glucose to Glucose-6-phosphate in the presence of ATP. Glucose-6-phosphate dehydrogenase plays a role in the subsequent oxidation of Glucose-6-phosphate in the presence of triphosphopyridine nucleotide (TPN). Hexokinase and Glucose-6-phosphate Dehydrogenase both serve as a tool in biochemical and analytical studies for determining glucose, fructose, mannose, ATP, and creatin-kinase activity. |
| General description: | Research area: Cell signaling Hexokinase and Glucose-6-phosphate Dehydrogenase are intracellular enzymes found distributed widely in the living cells. |
| General description: | Enzyme Commission numbers 2.7.1.1 + 1.1.1.49 |
| Other Notes: | Hexokinase Unit Definition: One unit will phosphorylate 1.0 μmole of |
| Other Notes: | Mixture of hexokinase and glucose-6-phosphate dehydrogenase |
| Packaging: | 500 units in glass bottle |
| Packaging: | 5000 units in poly bottle |
| Packaging: | Package sizes are based on hexokinase units. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | glucose-6-phosphate dehydrogenase activity: ≥75 units/mg protein (biuret); hexokinase activity: ≥65 units/mg protein |
| Storage Temp. | −20°C |
| UNSPSC | 12352204 |