Transforming Growth Factor-β2 human
SIGMA/H8666 - TGF-β2, recombinant, expressed in HEK 293 cells, HumanKine®, suitable for cell culture
Synonym: TGF-β2
MDL Number: MFCD00163957
Product Type: Chemical
TGF-beta Signaling
Research areas: Apoptosis, Differentiation, Epithelial Mesenchymal transition, Cancer biology, Bone remodelling, Developmental biology, Inflammation, Metabolism
Disease areas: Gastrointestinal cancers, Pulmonary, liver and renal fibrosis, Diabetes, Tuberculosis
See also: T7039; H8541; B3555; 11412272001; H4791; H8791
Research areas: Apoptosis, Differentiation, Epithelial Mesenchymal transition, Cancer biology, Bone remodelling, Developmental biology, Inflammation, Metabolism
Disease areas: Gastrointestinal cancers, Pulmonary, liver and renal fibrosis, Diabetes, Tuberculosis
See also: T7039; H8541; B3555; 11412272001; H4791; H8791
| assay | ≥95% (SDS-PAGE) |
| biological source | human |
| form | lyophilized powder |
| impurities | ≤1 EU/μg |
| mol wt | dimer 25 kDa (non-glycosylated) |
| packaging | pkg of 5 μg |
| potency | ≤0.5 ng/mL EC50 |
| quality | endotoxin tested |
| Quality Level | 200  |
| recombinant | expressed in HEK 293 cells |
| storage condition | avoid repeated freeze/thaw cycles |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P61812  |
| Analysis Note: | The specific activity was determined by the dose-dependent inhibition of IL-4 induced proliferation of mouse HT-2 (BALB/c spleen activated by sheep erythrocytes in the presence of IL-2). |
| Biochem/physiol Actions: | TGF-β2 is important for immune homeostasis by balancing lymphocyte proliferation, apoptosis, hematopoiesis, and embryogenesis. TGF-β2 is crucial in cell growth, differentiation, and survival. TGF-β2 is a strong growth inhibitor for normal and transformed epithelial, lymphoid, fibroblast, and keratinocyte cells. TGF-β is associated with the process of tumor development, progression and metastasis. TGF-β2 is a tumor suppressor in the early stages of carcinogenesis, but in the later stages acts as a tumor promoter by inducing epithelial-mesenchymal transition and stimulating angiogenesis. TGF-β2 inhibits antitumor action of NK (natural killer) cells, T-cells, macrophages, monocytes and neutrophils. TGF-β is also associated with inflammation and wound healing. The TGF-β gene is overexpressed in glioma tumors and also in colon cancer, gastric cancer and cervical lesions. |
| Biochem/physiol Actions: | Transforming growth factor-β2 (TGF-β2), like TGF-β1, is produced by many cell types and reported to be most concentrated in mammalian platelets. |
| General description: | Transforming growth factor-β2 (TGF-β2) belongs to the TGF-β superfamily. All threes isoforms (TGF-β1, TGF-β2 and TGF-β3) are known to share similar structure and function. The TGFB2 gene is mapped to human chromosome 1q41 and is localized to extracellular matrix. The active form of TGF-β precursor is produced upon proteolytic cleavage. TGF-β1 and TGF-β2 have 70% amino acid sequence identity. |
| Legal Information: | HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc |
| Preparation Note: | HumanKine TGF-β2 is expressed in human HEK 293 cells as a mature, non-glycosylated, disulfide-linked homodimer with a predicted molecular mass of approx. 25 kDa. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥95% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |